FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768966
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83181.0817123253823966No Hit
GATTATTACTGTGCAGCATTGGATGACAGCCTGAATGGTCCGGTATTCGG40770.5301924922558344No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGGTCACCATCTC35720.464519887745362No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32440.42186520600390653No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27380.35606255673202714No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGGCTGTCATCCAATG26090.3392867825105401No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCT23880.31054689023962045No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23050.29975317504284976No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG22300.28999981793733404No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20700.2691926561122338No Hit
GTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGGTCAC20600.2678922084981651No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20210.2628204628032969No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19820.2577487171084287No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18030.23447070481659787No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17720.2304393172129847No Hit
CCCCAGAGGGCAGCAGATCATCATAATAGATGAGGAGTTTGGGAGGCTTTCCTGGGAGCTGCTGGTACCAGTTTA17250.22432721342686154No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17050.22172631819872402No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16990.22094604963028272No Hit
GCATTGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16470.21418372203712518No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGG16440.21379358775290455No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15600.20286982779472695No Hit
CTCCTGTTCTGGAAGCAGGTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGCCTCC15520.20182946970347193No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15460.2010492011350307No Hit
GGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGT15450.2009191563736238No Hit
CATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14840.19298642592780435No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14710.19129584402951494No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14590.18973530689263243No Hit
CCTCTGGGGTCTCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT14360.1867442773802743No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14260.18544382976620552No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14020.1823227554924405No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13940.18128239740118549No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13760.1789415916958617No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13570.17647074122913106No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13340.17347971171677293No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12810.1665873393622085No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGT12690.16502680222532595No Hit
GGCAGAGGGTCACCATCTCCTGTTCTGGAAGCAGGTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAG11980.15579362416543774No Hit
GTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC11940.15527344511981023No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATTGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGAC11620.1511120127547902No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11440.14877120704946645No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11130.14473981944585326No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11070.143959550877412No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGC10950.1423990137405295No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10930.14213892421771573No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10870.14135865564927447No Hit
TCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAG10700.13914789470535757No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10400.1352465518631513No Hit
AGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGG10070.13095507473672438No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10000.13004476140687626No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9930.12913444807702812No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9920.12900440331562124No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9800.1274438661787387No Hit
GGGTCACCATCTCCTGTTCTGGAAGCAGGTCCAACATCGGAAATAATGCT9710.12627346332607683No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9710.12627346332607683No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGC9690.12601337380326308No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGGTCAC9560.1243227919049737No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9490.12341247857512556No Hit
CCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTC9300.12094162810839491No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGGTC9230.12003131477854678No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG9120.11860082240307114No Hit
CCCTCGGTGTCTGAAGCCCCCAGGCAGAGGGTCACCATCTCCTGTTCTGG9050.11769050907322301No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG8980.11678019574337486No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGAC8950.11639006145915425No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8870.11534970336789924No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8860.11521965860649235No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGGC8780.11417930051523734No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8710.11326898718538922No Hit
GCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGCCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCT8710.11326898718538922No Hit
GTTCTGGAAGCAGGTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGCCTCCCAAA8700.11313894242398234No Hit
CTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG8690.11300889766257546No Hit
GTGCAGCATTGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGACC8630.11222862909413421No Hit
TCATAATAGATGAGGAGTTTGGGAGGCTTTCCTGGGAGCTGCTGGTACCA8570.11144836052569296No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC8460.11001786815021732No Hit
GTGCCAGACTTGGAGGCAGAGAATCGGTCAGAGACCCCAGAGGGCAGCAG8170.1062465700694179No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8160.10611652530801102No Hit
ATCCAATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC8130.10572639102379039No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8040.1045559881711285No Hit
CCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAG7940.10325554055705974No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC7880.10247527198861849No Hit
ATACCGGACCATTCAGGCTGTCATCCAATGCTGCACAGTAATAATCAGCC7870.10234522722721161No Hit
GCTTGGTCCCTCCGCCGAATACCGGACCATTCAGGCTGTCATCCAATGCTGCACAGTAATAATCAGCCTCATCCT7770.10104477961314284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)