FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498602
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30880.6193316512970265No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC21030.4217792949085643No Hit
CCTCTGAGGAGCCTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20060.4023249004215787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19900.39911592813506563No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14430.28940918808989935No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13540.2715592797461703No Hit
GACTACTACTGTCAGTCTTATGATAAATTTGTGATATTCGGCGGAGGGAC13400.26875142899547133No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGTCCGTAACCATCTCCTGCACCCGCAGCAGTGGCAGTATTGCCAG12930.25932507290383916No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC12290.24648918375778675No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12260.24588750145406557No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12060.24187628609592418No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCCTCAAGCCAACAAGG11920.23906843534522523No Hit
CTACTACTGTCAGTCTTATGATAAATTTGTGATATTCGGCGGAGGGACCA11660.23385385537964148No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11580.23224936923638495No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11160.22382581698428805No Hit
GTGTCGGAGTCTCCGGGGAAGTCCGTAACCATCTCCTGCACCCGCAGCAGTGGCAGTATTGCCAGCAACTATGTG11150.22362525621638102No Hit
GTAGTAGTCCGCCTCGTCCTCAGTCTTCAGTCCAGAGATAGTGAGGGAGG10990.22041628392986792No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10920.21901235855451845No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCGGACTACTACTGTCAGTCTTAT10880.21821011548289015No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAAATTTATCATAAGACTGACAGTAGTAGTCCGCCT10620.21299553551730638No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCCTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9740.1953461879414844No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGTCCGTAAC9490.19033216874380768No Hit
CAGTAGTAGTCCGCCTCGTCCTCAGTCTTCAGTCCAGAGATAGTGAGGGA9480.19013160797590062No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8860.17769684036566238No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8570.1718805780963574No Hit
GTCCAGAGATAGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA8140.1632564650763535No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8020.16084973586146867No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8020.16084973586146867No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7930.15904468895030505No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7860.15764076357495557No Hit
GTATTATTGTGCGAGAGATACGGGTGTCGAACTTGACTACTGGGGCCAGG7810.15663795973542022No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7710.15463235205634956No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7670.15383010898472127No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7650.15342898744890715No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGTCC7640.1532284266810001No Hit
GTTCCCGCCCTCCTCTGAGGAGCCTCAAGCCAACAAGGCCACACTGGTGT7560.15162394053774353No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7450.1494177720907658No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7430.14901665055495167No Hit
CAATAATACATAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGAAGATA7350.1474121644116951No Hit
CACTATCTCTGGACTGAAGACTGAGGACGAGGCGGACTACTACTGTCAGTCTTATGATAAATTTGTGATATTCGG7070.14179646291029718No Hit
ATAAAAGACCCTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGACAGC6960.13959029446331944No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6630.1329717891223862No Hit
GATTATAATGGTGGGAGAACTGCCCGGGCGCTGCTGGTACCACTGCACATAGTTGCTGGCAATACTGCCACTG6560.13156786374703672No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6280.12595216224563882No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6120.12274318995912571No Hit
CACCAGTGTGGCCTTGTTGGCTTGAGGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6060.12153982535168331No Hit
GTCCGTAACCATCTCCTGCACCCGCAGCAGTGGCAGTATTGCCAGCAACT5910.11853141383307729No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT5860.11752860999354195No Hit
GGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5810.1165258061540066No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC5780.1159241238502854No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5660.11351739463540057No Hit
GTCTGAGAGTCGAGGACACGGCTATGTATTATTGTGCGAGAGATACGGGT5600.11231403002795817No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGC5580.11191290849214403No Hit
CCTCCAACTCTGCCTCCCTCACTATCTCTGGACTGAAGACTGAGGACGAG5580.11191290849214403No Hit
CTCCTGTGCAGTCTCTGGAATCACCGTCACTAACAACTACATGAACTGGGTCCGCCAGGCTCCCGGGAAGGGG5570.11171234772423695No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5490.11010786158098043No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5430.108904496973538No Hit
GCTTGGTCCCTCCGCCGAATATCACAAATTTATCATAAGACTGACAGTAG5390.10810225390190974No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAAATTTAT5340.10709945006237441No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT5330.10689888929446734No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5320.10669832852656026No Hit
ATCTCTGGACTGAAGACTGAGGACGAGGCGGACTACTACTGTCAGTCTTA5320.10669832852656026No Hit
CCTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACTATCTCTGGA5310.10649776775865319No Hit
GTCTTCAGTCCAGAGATAGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAGCGATCAGGGACC5270.10569552468702491No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5240.10509384238330371No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5140.10308823470423303No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5080.10188487009679062No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAGGGTCTTTTATTATCTTCAAAGATT5040.10108262702516237No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCCTCAAGCCAACAAGGCC5040.10108262702516237No Hit
ATGTATTATTGTGCGAGAGATACGGGTGTCGAACTTGACTACTGGGGCCA5030.10088206625725529No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5010.10048094472144115No Hit
ATAATACATAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGAAGATATA5000.10028038395353409No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAGGCTCCTCAGAGGA4990.10007982318562701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA200.006559031553.27343858
TAGTTCG200.006610709753.16784757
ACGCGTC200.006610709753.16784757
CGTTGGT200.007703215351.1508424
GGTATCA5050.047.9337461
GTATAAA350.001427848138.964251
GACAGGT655.972124E-736.716311
CTTAGAT400.002250621635.51562558
GTATCAA10400.033.7658921
CAGTAGT13250.030.95608570
GCAATTT500.00455430330.756168
GTAAAAC450.00490783430.290333
CTCAGCA3750.029.9907970
ACTGGTG14850.029.40273570
GTATTAG704.1704625E-529.2231881
ACGGGTA704.1790357E-529.21439416
GCACCAT704.1876236E-529.2056056
AATGGTA1853.647965E-728.60806570
ATCAACG12550.028.5103233
ACAGGAC605.8066635E-428.4114321
Produced by FastQC (version 0.11.5)