FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297772
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50100.3860462392469555No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43590.3358833446861236No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40010.3082976054345447No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34540.26614844518143405No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32650.2515850241799022No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31940.24611410941213094No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30190.23262946033663848No Hit
ATGTAGATCAGGAGCTTGGGGGCTTTCCCGGGTTTTTGCTGATACCAATT29570.22785204180703544No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29480.22715854556886725No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28470.21937597667386877No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28380.2186824804357006No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27790.21413622731882026No Hit
GATCTACATTGCGGCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGAACAGATTTCA27720.21359684135580054No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27540.21220984887946417No Hit
GTAATATATGGCCGTGTCTGCGGCGGTCACAGAGCTCAACCTCAGGCCGA26470.20396494915902022No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25460.19618238026402174No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25360.1954118288882793No Hit
ATTATACCCCACTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAACCGA24780.19094263090897323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23690.18254362091338078No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTTCCAGATCCACTGCCA23340.1798466910982823No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23310.17961552568555955No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22770.17545454825655046No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCACTTACTTAAATTGGTATCAGCAAAAA22230.17129357082754137No Hit
ACTTACTACTGTCAACAAAGTTATTATACCCCACTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAACCGAA21990.16944424752575954No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21050.16220106459378072No Hit
GTTATTATACCCCACTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAC20850.16065996184229586No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20250.1560366535878413No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20170.15542021248724736No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19920.1534938340478913No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19860.15303150322244585No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCA19490.15018046313219888No Hit
GTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG19320.14887052579343676No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19160.14763764359224885No Hit
ATATATTACTGTGCGAGAACAACCGTATCGGCGGCTGGCACTTTCGCTTT19030.1466359268037837No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18470.14232083909962612No Hit
GGGTATAATAACTTTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAAT17850.13754342057002308No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17280.13315127772829125No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCC17220.1326889469028458No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17140.13207250580225185No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16640.12821974892353974No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16550.12752625268537154No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16500.12714097699750032No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16300.12559987424601546No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16050.12367349580665941No Hit
GTGCTAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA16030.12351938553151091No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15610.12028306975339273No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15510.11951251837765031No Hit
CCTTCGGCGGAGGGACCAAGGTGGAGATCAACCGAACTGTGGCTGCACCA15450.11905018755220485No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15440.11897313241463062No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15320.11804847076373971No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15250.11750908480072No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14950.11519743067349272No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGCCGCAATGTAGATCAGGAGCTTGGGGGCTTTCCCGGGTTTTTG14900.1148121549856215No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14870.11458098957289878No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14860.11450393443532454No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14820.11419571388502758No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14660.11296283168383968No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14630.11273166627111696No Hit
GTATAATAACTTTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAAT14440.11126761865720636No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14370.11072823269418666No Hit
GACCAAGGTGGAGATCAACCGAACTGTGGCTGCACCATCTGTCTTCATCT14280.11003473645601848No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14230.10964946076814726No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14180.10926418508027604No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14140.10895596452997908No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT14130.10887890939240484No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14120.10880185425483058No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14040.10818541315423666No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14040.10818541315423666No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13940.10741486177849421No Hit
GATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAAAGTTATTATACC13630.1050261525136927No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13610.10487204223854421No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13600.10479498710096997No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13540.10433265627552452No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13520.10417854600037603No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACCTCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGAAGGGT13370.10302271893676239No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13310.10256038811131693No Hit
GAGTGAAATCTGTTCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA13230.10194394701072299No Hit
GTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAAAGTTATTATACCCCACTCACCTTCGGCGGAGGGA13040.10047989939681239No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13000.10017167884651543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)