FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297772
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44070.33958199128968725No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41450.3193935452452357No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39440.3039054625928129No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36140.27847726719331284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32490.2503521419787143No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30270.23324590143723242No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29810.22970136510881725No Hit
GATCTACATTGCGGCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGAACAGATTTCA27690.21336567594307781No Hit
ATGTAGATCAGGAGCTTGGGGGCTTTCCCGGGTTTTTGCTGATACCAATT27070.20858825741347478No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26990.20797181631288086No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26960.20774065090015814No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26020.2004974679681793No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25790.19872519980397174No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25770.19857108952882324No Hit
GTAATATATGGCCGTGTCTGCGGCGGTCACAGAGCTCAACCTCAGGCCGA25360.1954118288882793No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24470.1885539216441717No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24120.1858569918290732No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24020.18508644045333078No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22840.17599393421957016No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22240.1713706259651156No Hit
ATTATACCCCACTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAACCGA22230.17129357082754137No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCACTTACTTAAATTGGTATCAGCAAAAA21540.16597676633491862No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTTCCAGATCCACTGCCA21220.16351100193254287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21010.16189284404348375No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20860.1607370169798701No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20780.16012057587927617No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20440.1575007012017519No Hit
ACTTACTACTGTCAACAAAGTTATTATACCCCACTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAACCGAA20360.156884260101158No Hit
GTTATTATACCCCACTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAC20300.15642192927571252No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCA20120.15503493679937616No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19770.15233800698427766No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19270.14848525010556554No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19130.14740647817952612No Hit
GTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG19090.14709825762922918No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19010.14648181652863523No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18240.14054857093541856No Hit
ATATATTACTGTGCGAGAACAACCGTATCGGCGGCTGGCACTTTCGCTTT17710.1364646486439837No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17670.13615642809368672No Hit
GGGTATAATAACTTTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAAT17190.13245778149012308No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17040.13130195442650944No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16970.13076256846348974No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16940.13053140305076702No Hit
GTGCTAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA16930.13045434791319277No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16310.12567692938358974No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGAACAGATTTCACT16180.12467521259512455No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15720.12113067626670941No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCC15630.12043718002854123No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15420.11881902213948213No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15290.11781730535101699No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15110.1164303128746806No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT15050.11596798204923514No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14600.11250050085839422No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14440.11126761865720636No Hit
GAGTGAAATCTGTTCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA14420.11111350838205786No Hit
GATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAAAGTTATTATACC14410.11103645324448362No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14350.11057412241903816No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14250.10980357104329574No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14130.10887890939240484No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGCCGCAATGTAGATCAGGAGCTTGGGGGCTTTCCCGGGTTTTTG14120.10880185425483058No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14060.10833952342938513No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACCTCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGAAGGGT13910.10718369636577149No Hit
CCTTCGGCGGAGGGACCAAGGTGGAGATCAACCGAACTGTGGCTGCACCA13800.10633608985245481No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13750.10595081416458361No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13690.10548848333913816No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13690.10548848333913816No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13640.10510320765126693No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13490.10394738058765329No Hit
GTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAAAGTTATTATACCCCACTCACCTTCGGCGGAGGGA13170.10148161618527753No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG13130.10117339563498057No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13090.10086517508468361No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13020.10032578912166389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAA200.006085026554.29841260
TACGTAT400.00777677951.02971370
GTGGTAT3300.043.9854741
TTAACGG350.001415296939.0389835
GTATCAA20150.036.8555071
TATCAAC22350.032.6172452
GTACGTA550.003503684232.45281269
ATCAACG23050.031.9195383
TCAACGC23200.031.7131614
CAACGCA23650.031.110945
AACGCAG24000.030.6607846
AATGGTA5800.030.50051770
ACGCAGA25300.029.0898117
CGCAGAG25450.028.9228178
AGAGTAC25700.028.65030311
GCAGAGT26200.028.2259279
CTCTATC606.159857E-428.1302431
ATTCACT2200.027.6134622
CGTGTCT8000.027.4428513
GATTCAC2100.027.326521
Produced by FastQC (version 0.11.5)