FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044473
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30040.2876091579198313No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27780.26597145163158836No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27480.2630991897349189No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25790.2469187810503479No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23760.22748314221621813No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21210.20306891609452807No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21140.20239872165197184No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18370.17587817013939086No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18340.17559094394972394No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17390.1664954479436041No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16730.16017647177093136No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16680.1596977614548198No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16540.15835737256970742No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATAAAACGAACTGTGGCTGCACCA16510.15807014638004047No Hit
GGCTAAAAACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATAAAACGAACTGTGGCTGCACCAT15680.15012355513258838No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15670.15002781306936608No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13890.13298572581579418No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13860.13269849962612726No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13790.13202830518357103No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13510.12934752741334626No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA13510.12934752741334626No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12350.11824144807955782No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12110.1159436385622223No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGGA12020.11508195999322146No Hit
GTCTGGGACCCCGGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGATA12000.11489047586677684No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11510.11019911476888346No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11450.10962466238954956No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10970.10502904335487849No Hit
TTATTACTGCGTGCAGGCTAAAAACTGGCCGTACACTTTTGGCCAGGGGA10880.10416736478587767No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10830.1036886544697661No Hit
GTGCAGCCACAGTTCGTTTTATCTCCAGCTTGGTCCCCTGGCCAAAAGTG10800.10340142828009916No Hit
GTAATAAATCCCAACATCCTCAGCCTCCACCCTGCTGATCTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG10670.10215678145820907No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10650.10196529733176443No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10610.1015823290788752No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10550.1010078766995413No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10480.10033768225698511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG200.006773113752.8467849
CCGTTTA200.00810218650.5054661
GTGGTAT4950.033.3302041
GGTATCA24000.031.2853321
ATCAACG45350.030.4754263
GTATCAA47650.030.384541
TCAACGC46000.030.2583034
CAACGCA46100.030.1211815
AACGCAG47000.029.5429786
CGCAGAG49750.028.0451078
AGAGTAC50700.027.45197911
ACGCAGA50800.027.399257
CAGAGTA51150.027.277510
GCAGAGT51900.027.1424319
TATCAAC53500.026.7949622
TGTTAGA16850.026.68550370
ACGGGTC1655.456968E-1226.4900195
ATTCACT3400.025.733082
TAGTCAC1359.962605E-925.42245738
GTGTAGA1651.5643309E-1024.4874971
Produced by FastQC (version 0.11.5)