FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041063
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41650.40007184963830233No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39140.37596187742720666No Hit
GTATTACTGTGCGAGAGATTACGATAACTGGAACTACGGGGAGTTTGACTACAGGGGCCAGGGAACCCTGGTCAC36510.35069923722195484No Hit
GGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTACTCGGGCC34030.32687743200939806No Hit
GTAATACAGAGCCGTGTCTTCGGCTCTCAGGCTGTCCATTCGCAGATATA33660.3233233723607505No Hit
CAGTAATACAGAGCCGTGTCTTCGGCTCTCAGGCTGTCCATTCGCAGATA32460.3117966924191908No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28010.2690519209692401No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26460.2541632927113921No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24990.24004310978298143No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTCGTCAGAGCCTCCTG24890.23908255312118482No Hit
GTAATAAACCCCAACATCCTCAGGTTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA23820.22880459683996068No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23450.2252505371913131No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23180.22265703420446215No Hit
CTACAAACTCCCCTCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCAT22720.2182384735601976No Hit
CTGTATTACTGTGCGAGAGATTACGATAACTGGAACTACGGGGAGTTTGA21920.21055402026582448No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21850.2098816306025668No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20320.19518511367707814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20310.1950890580108985No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20020.19230344369168823No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20000.19211133235932887No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19490.187212493384166No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19380.1861558810561897No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19060.18308209973844042No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18990.18240971007518278No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18980.1823136544090031No Hit
GTACCTGCAGAGGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTACTCGGGCCTCCGGGGTCCCTGA18680.17943198442361316No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18570.17837537209563686No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18130.17414892278373162No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA17760.17059486313508404No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG17660.1696343064732874No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17390.16704080348643646No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAACCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCTCTACAAACT17280.16598419115846014No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT17120.1644473004995855No Hit
GCCCTGGCCTCTGCAGGTACCAATCCAAATGGTTGTATCCATCACGATGC16660.16002873985532096No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16560.1590681831935243No Hit
GAGTTTGTAGAGCCTGCATGCAGTAATAAACCCCAACATCCTCAGGTTCCACTCTGCTGATTTTCAGTGTAAAAT15890.1526324535594868No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15840.15215217522858848No Hit
GGATACAACCATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTACT15650.15032711757117487No Hit
CTCCTGCATCGTGATGGATACAACCATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTCCACAGCTCCTGAT15610.14994289490645618No Hit
GTACCTATAGCATGCACTGGGTCCGCCAGGCTCTAGGGAAGGGGCTGGAG15580.14965472790791717No Hit
GTTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA15450.14840600424758155No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15220.14619672392544927No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC15190.1459085569269103No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAAATGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC15130.14533222292983228No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAAATGTGCTGACTCAGTCTCCACTCTCC14870.14283477560916102No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14570.13995310562377108No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14510.1393767716266931No Hit
GTTTATTACTGCATGCAGGCTCTACAAACTCCCCTCTTCGGCGGAGGGAC14420.13851227063107613No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAAATGTGCTGA14410.13841621496489648No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAGAGG14170.13611087897658453No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCCTCTGCAGGTA14160.13601482331040485No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG13900.13351737598973357No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC13760.13217259666321826No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAGCATGCACTGGGTCCGCCAGGCTCTAGGGAAGGGG13660.13121204000142161No Hit
GAGTGGAACCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCTCTACAA13480.12948303801018768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13390.12861853701457068No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13390.12861853701457068No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13320.12794614735131304No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG13270.1274658690204147No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13210.12688953502333672No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13190.12669742369097742No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13090.12573686702918074No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13030.12516053303210278No Hit
CTGCAGGTACCAATCCAAATGGTTGTATCCATCACGATGCAGGAGGCTCTGACGAGACCTGCAGGAGATGGAGGC12990.1247763103673841No Hit
CAGTAATAAACCCCAACATCCTCAGGTTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12920.12410392070412646No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12780.12275914137761115No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12700.12199069604817384No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12550.12054986105547888No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12490.11997352705840089No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12460.11968536005986188No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12430.11939719306132289No Hit
CATTAGCATAGTTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG12210.11728396840537028No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12150.1167076344082923No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAAATGTGCTGACTCAGTCTCCACT12130.11651552307593296No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12050.11574707774649565No Hit
CACTATATCTGCGAATGGACAGCCTGAGAGCCGAAGACACGGCTCTGTATTACTGTGCGAGAGATTACGATAA11920.11449835408616No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11820.11353779742436336No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11800.11334568609200404No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11760.11296146342728537No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11530.1107521831051531No Hit
GAGTAGTGGTGATCACATATATTATGCAGACTCAGTGAAGGGGCGATTCACCATCTCCAGAGACAACGCCCGGAA11420.1096955707771768No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11360.1091192367800988No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11230.10787051311976316No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG11150.10710206779032586No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11100.10662178945942753No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11050.1061415111285292No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG11040.10604545546234953No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11010.10575728846381055No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10950.10518095446673256No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10950.10518095446673256No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10890.10460462046965456No Hit
GTGATGGATACAACCATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTT10790.10364406380785794No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10670.10249139581370195No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTC10650.10229928448134264No Hit
GCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAGAGGGGAGT10620.10201111748280364No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10510.10095450515482732No Hit
TTATTACTGCATGCAGGCTCTACAAACTCCCCTCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGG10420.10009000415921035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATG200.004926664357.27373568
ACTTCGT200.007390334751.694138
GTATGGG400.00924034248.8500570
TTGCTTA306.0435187E-446.40622348
TCTGGAG11750.041.02018770
GGTATCA7800.039.4743161
CGTATGC901.200533E-1038.39039641
TCGTATG901.2187229E-1038.35132240
CCTATTA451.05323415E-438.0068242
GTATCAA18100.036.1011921
ATCTCGT1151.8189894E-1235.93502837
GTGGTAT2150.035.006681
TGGTATC2400.032.7808882
TGTTAGA10950.032.7154170
AATGGTA2559.094947E-1230.65101470
ATGATCT1359.094947E-1230.55074334
TATCAAC21300.030.351932
ATCAACG21850.029.5879253
CGGTACC604.610496E-429.55718460
GATCTCG1401.4551915E-1129.49666236
Produced by FastQC (version 0.11.5)