FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041063
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39830.38258971839360345No Hit
GTATTACTGTGCGAGAGATTACGATAACTGGAACTACGGGGAGTTTGACTACAGGGGCCAGGGAACCCTGGTCAC35180.3379238336200595No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32890.3159270860649164No Hit
GGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTACTCGGGCC30250.29056839019348496No Hit
GTAATACAGAGCCGTGTCTTCGGCTCTCAGGCTGTCCATTCGCAGATATA29590.28422871622562706No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29080.2793298772504642No Hit
CAGTAATACAGAGCCGTGTCTTCGGCTCTCAGGCTGTCCATTCGCAGATA28670.2753915949370979No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTCGTCAGAGCCTCCTG27290.2621359130043043No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24030.23082176582973363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23840.22899670817232No Hit
GTAATAAACCCCAACATCCTCAGGTTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA23720.22784404017816406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23540.2261150381869301No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23440.22515448152513345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20660.19845100632718674No Hit
CTGTATTACTGTGCGAGAGATTACGATAACTGGAACTACGGGGAGTTTGA20590.1977786166639291No Hit
CTACAAACTCCCCTCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCAT20300.1949930023447188No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19110.18356237806933876No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19090.18337026673697943No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19070.1831781554046201No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18830.18087281941630814No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18250.1753015907778876No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18220.17501342377934861No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17660.1696343064732874No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17060.16387096650250754No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16940.16271829850835157No Hit
GAGTTTGTAGAGCCTGCATGCAGTAATAAACCCCAACATCCTCAGGTTCCACTCTGCTGATTTTCAGTGTAAAAT16820.1615656305141956No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16800.16137351918183626No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16720.16060507385239894No Hit
GTACCTGCAGAGGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTACTCGGGCCTCCGGGGTCCCTGA16630.15974057285678198No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16570.159164238859704No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16550.15897212752734466No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA16460.15810762653172766No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG16070.15436145555072076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15990.15359301022128344No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAACCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCTCTACAAACT15950.15320878755656478No Hit
GCCCTGGCCTCTGCAGGTACCAATCCAAATGGTTGTATCCATCACGATGC15910.15282456489184612No Hit
CTCCTGCATCGTGATGGATACAACCATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTCCACAGCTCCTGAT15780.1515758412315105No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT15470.1485981155799409No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15400.14792572591668324No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15340.14734939191960525No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15040.1444677219342153No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAAATGTGCTGACTCAGTCTCCACTCTCC14820.1423544972782627No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAAATGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC14730.14148999628264572No Hit
GGATACAACCATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTACT14600.14024127262231006No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14540.1396649386252321No Hit
GTTTATTACTGCATGCAGGCTCTACAAACTCCCCTCTTCGGCGGAGGGAC14350.13783988096781846No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG14310.1374556583030998No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14270.13707143563838114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC13930.13380554298827257No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAGCATGCACTGGGTCCGCCAGGCTCTAGGGAAGGGG13900.13351737598973357No Hit
GTACCTATAGCATGCACTGGGTCCGCCAGGCTCTAGGGAAGGGGCTGGAG13880.13332526465737427No Hit
GAGTGGAACCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCTCTACAA13870.1332292089911946No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTC13860.1331331533250149No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAGAGG13740.13198048533085893No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13030.12516053303210278No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13020.1250644773659231No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAAATGTGCTGA12900.12391180937176711No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12880.12371969803940781No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCCTCTGCAGGTA12760.12256703004525182No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12750.12247097437907216No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12710.12208675171435351No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12680.1217985847158145No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12640.12141436205109586No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12600.12103013938637719No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG12530.12035774972311954No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12380.11891691473042458No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAAATGTGCTGACTCAGTCTCCACT12270.11786030240244826No Hit
GTTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12220.11738002407154995No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC12210.11728396840537028No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12080.11603524474503464No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11770.11305751909346505No Hit
CAGTAATAAACCCCAACATCCTCAGGTTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11500.11046401610661409No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11300.10854290278302081No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11180.10739023478886485No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11130.10690995645796651No Hit
CTGCAGGTACCAATCCAAATGGTTGTATCCATCACGATGCAGGAGGCTCTGACGAGACCTGCAGGAGATGGAGGC11110.1067178451256072No Hit
CACTATATCTGCGAATGGACAGCCTGAGAGCCGAAGACACGGCTCTGTATTACTGTGCGAGAGATTACGATAA11100.10662178945942753No Hit
GAGTAGTGGTGATCACATATATTATGCAGACTCAGTGAAGGGGCGATTCACCATCTCCAGAGACAACGCCCGGAA11020.10585334412999022No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10950.10518095446673256No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10910.1047967318020139No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10850.10422039780493592No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10730.10306772981077995No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10730.10306772981077995No Hit
GGCCTATCCAGTAGGTGATCAGCACTGAGCACCGAGGATTCACCATGGAA10620.10201111748280364No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10620.10201111748280364No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10610.10191506181662396No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10570.10153083915190532No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10560.10143478348572565No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10550.10133872781954599No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10520.10105056082100698No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10510.10095450515482732No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10490.100762393822468No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10430.10018605982539No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10420.10009000415921035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC200.0068564452.68369354
CAGAACG200.007180870552.07135845
GGTATCA7550.042.0925181
CGGTACC459.656363E-538.57137745
TAACGGC451.0141079E-438.2519436
TCTGGAG10900.035.4961570
TTAACGG501.893817E-434.3984335
GAGGGTT1002.2683002E-433.35414570
GTATCAA18150.032.9480971
AATGGTA2900.032.20470
TATCAAC19950.029.7954542
ACGGGGC1052.5669578E-829.27892516
ACGGGTC704.1276242E-529.2718935
TATGGTT1155.1469036E-429.00360570
CAACGCA20700.028.871235
TCAACGC20650.028.6062564
ATCAACG20550.028.5806753
GTGTGTA605.7336234E-428.4765681
CAATACT605.759473E-428.4546814
GTGGTAT2900.028.2801781
Produced by FastQC (version 0.11.5)