FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282248
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA52970.4131026135349792No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG41620.32458619549416334No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33760.26328760115047944No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA31380.24472644917363878No Hit
GTTTATTACTGTGCGAGGGCGGTCTCAAGATGGCCCAACTGGTTCGACCC30340.2366156936879605No Hit
GTCTCTGGTGGCTCCATCAGCACTAGTAGTGACTACTGGGGCTGGATCCG28580.22288979978912038No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26740.20854000162215108No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24720.19278641885189135No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23920.18654737617060038No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC23890.18631341207005198No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23630.1842857231986324No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG23340.18202407022666442No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT23050.17976241725469644No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT22210.1732114224393409No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21990.1714956857019859No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21830.17024787716572767No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG20700.16143522937840415No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCATCCAGG20000.15597606703227457No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19550.1524666055240484No Hit
GATTATTACTGCGTAACATGGGATAGCAGCCTGGATGCTGTGGTATTCGG19440.15160873715537088No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18910.1474753713790156No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT18680.14568164660814445No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18610.14513573037353147No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18450.1438879218372733No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17830.13905266375927278No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17570.1370249748878532No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCACTAGTAGTGACTACTGGG17080.13320356124556246No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16960.13226770484336883No Hit
CTATTATAGTGGGAGCACCTACTACCACCCGTCTCTCAAGAGTCGAGTCACCATATCCGTAGACACGGCCAAGAA16790.1309419082735945No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG16570.12922617153623947No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16200.1263406142961424No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC16190.12626262626262627No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC15940.12431292542472283No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15690.12236322458681939No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15430.12033553571539983No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14790.11534430157036704No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14760.11511033746981862No Hit
ACATGGGATAGCAGCCTGGATGCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14640.11417448106762497No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT14540.11339460073246362No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14520.11323862466543133No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14400.11230276826323768No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14150.11035306742533425No Hit
TCTTGAGACCGCCCTCGCACAGTAATAAACAGCCGTGTCTGCGGCGGTCA14030.1094172110231406No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA14020.10933922298962448No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC13920.1085593426544631No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC13860.10809141445336627No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13850.10801342641985014No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13810.10770147428578558No Hit
CTATAATAGATATTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG13720.10699958198414035No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG13670.10660964181655966No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13340.10403603671052715No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT13300.1037240845764626No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13270.10349012047591417No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13240.10325615637536577No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13200.10294420424130121No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAGCATTGGGAATAATTATGTATCCTGGTTCCAGCAG13190.10286621620778508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT13950.059.1059470
GGATGGG16050.041.26640370
AATGGTA5250.041.19421870
GGTATCA9300.038.7336351
CTACGGA14400.038.31089469
GTAGTTT901.13103E-437.5468170
GTATCAA21350.036.927971
GTGTCTA13600.034.7565665
TCTACGG15050.033.00409368
GTCTACG14900.032.54826767
TGGTATC3450.032.494342
GGCCGTG15100.031.64719461
TATCAAC24700.031.495712
TTGGCCG14950.031.3212759
TTAACGG553.3823954E-431.16256735
AGTGGTT15050.031.04296751
TCAGGTC11450.030.88257412
TCTATAC553.566689E-430.8818453
AAGTGGT15200.030.6760150
GTATAGT902.2776476E-730.2073611
Produced by FastQC (version 0.11.5)