FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407857
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43320.3077017054999194No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42470.30166416049357286No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38970.2768036810556754No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38910.2763775014081686No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33960.24121768048885645No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32990.2343277761874963No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG32860.23340438695123156No Hit
GATTATTACTGCAGCTCATATACAACCAGCAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGT31980.22715375212113165No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30470.21642823099221015No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC30170.21429733275467608No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGGTTGTATATGAGCTG28250.20065958403445802No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27070.19227805096682404No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26990.19170981143681495No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26630.1891527335517741No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24890.17679352377407648No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT24080.1710400985327345No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATAACTATGTCT23520.16706242182267092No Hit
CTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGCCCCCAAAC22580.16038560734506416No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT22420.159249128285046No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCACGTAG22420.159249128285046No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG20820.1478843376848643No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20610.14639270891859046No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATAACTATGTCTCCTGGTACCAGCAGCACCCAGG20270.14397769091605184No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCACGTAGCTG19520.13865044532221668No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGTTCAACTTCAGGGAGA19460.13822426567470986No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTG19450.13815323573345872No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATA19410.1378691159684542No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCACGTAGCTGCTGG19040.13524100814216217No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18450.13105024160834516No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18220.12941655295956905No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGG17860.1268594750745282No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17150.12181634924569754No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16660.1183358821243919No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15980.11350584611931468No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15190.10789448076047496No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15040.10682903164170793No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14870.10562152264043863No Hit
TAGTTATAGTTATAACCACCAACGTCATTGCTGGTTCCAGTGCAGGAGAT14700.10441401363916931No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCACGTAGCTGCTGGCCGCGTACTT14560.10341959446165341No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14490.10292238487289547No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14270.10135972616537049No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14170.10064942675285915No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGGTTGTATATGAGCTGCAGTAATAAT14090.10008118722285005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.044.8730051
CTAACGC603.3481047E-739.5388873
GTATCAA36000.037.573951
CGTATGC3650.036.89851841
TCGTATG3800.036.28903240
TATACAC4250.034.2891853
CCTAACG701.1194625E-633.8904722
ATCAACG41500.032.747183
TCAACGC41700.032.4275784
ATGCCGT4200.032.24513244
TTATACA4650.032.0684052
CCGTCTT4250.032.0137547
CAACGCA42400.031.8922165
TATCAAC42650.031.7052732
CGAGACG4200.031.47419723
GACGGAC4100.031.44195426
GTCTACT5950.031.4177267
AACGCAG43150.031.337896
TCTCGTA3950.031.31915738
ATCTCGT3950.031.26173237
Produced by FastQC (version 0.11.5)