FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407857
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45650.324251681811434No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45340.32204975363264876No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT39800.2826991661795196No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37960.2696296569893107No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35920.25513954897407903No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34530.24526638714017118No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG33220.23596146483627245No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31810.2259462431198623No Hit
GATTATTACTGCAGCTCATATACAACCAGCAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGT31250.22196856640979873No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG30130.21401321298967152No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGGTTGTATATGAGCTG28030.19909692532693304No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27070.19227805096682404No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26060.18510402690045935No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25270.17949266154161964No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24820.17629631418531855No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24590.1746626255365424No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCACGTAG24340.17288687700526403No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATAACTATGTCT22220.1578285294600233No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC21860.1552714515749824No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT20740.14731609815485522No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTG20650.146676828683595No Hit
CTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGCCCCCAAAC20390.1448300502110655No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATAACTATGTCTCCTGGTACCAGCAGCACCCAGG19470.13829529561596102No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18830.13374937937588832No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCACGTAGCTGCTGG18770.1333231997283815No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18650.13247084043336788No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCACGTAGCTG18500.13140539131460083No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGTTCAACTTCAGGGAGA18360.13041097213708494No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATA18290.12991376254832698No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17730.1259360858382634No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17710.1257940259557611No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16070.1141451155905749No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGG15920.11307966647180787No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15770.11201421735304083No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT15690.11144597782303174No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15320.10881786999673972No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15070.10704212146546133No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCACGTAGCTGCTGGCCGCGTACTT15040.10682903164170793No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14580.1035616543441557No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14420.10242517528413753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG352.5435613E-548.3575942
CTAACGC405.605779E-542.3008653
GGTATCA16300.040.5202561
AGGTACG558.425715E-636.9276162
TACGTTA400.002450850334.90852447
GTATCAA38500.034.3106581
TGGTATC5750.033.555962
GTGGTAT5800.032.702821
ATCAACG43600.030.0374973
TCAACGC43900.029.8258724
GGTACGG803.2104035E-629.6106053
TATCAAC44450.029.5476322
CAACGCA44600.029.3587955
AACGCAG45100.029.033316
ACGGGTA4100.028.06301516
ACGCAGA47500.027.5663627
CGCAGAG47550.027.3950658
ATAGCGG2400.027.34280863
AATGGTA7400.027.1404570
TACCGGG500.008487252527.0802542
Produced by FastQC (version 0.11.5)