FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263142
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTACCACACCGAGGACGTTCGGCCAAGGGA66580.5270982993202664No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52800.4180052598995204No Hit
GTAATAGACAGCCGTGTCGTCAGTCCTCAGGCTGTTCATTTGCAGATACA47370.375017218966672No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43040.34073762094839694No Hit
CAGTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTGTGTCCCAGACCCACTGCCA42300.33487921389677483No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38780.3070121965701402No Hit
CTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTAGCCTGGCACC37210.2945828735011582No Hit
GTGTATTACTGTCAGCAGTATGCTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA36690.2904661550324508No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32810.25974910184286487No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32280.2555532157112977No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30700.243044724979456No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30290.23979885080220592No Hit
GTATGCTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT29710.23520712635633997No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCGGTGGCAGTGGGTCTGGGACACACTTCA29280.2318029168533704No Hit
GTTTACAGCAACTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCTCC28340.2243611565445532No Hit
CTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA27740.21961109677296775No Hit
GTCTATTACTGTGCGAGAGAGGGGGGGTCTGTTTATTATGCTTATAGCAGTGAGGGTTACCTTGATTATTGGGGC27680.21913609079580917No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26640.21090265385839438No Hit
GTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGA26170.20718177370398577No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGACTGTTTACAG26040.20615259408680894No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25970.20559842044679064No Hit
GTGTGGTAGCATACTGCTGACAGTAATACACTGCAAAGTCCTCAGGCTCC25710.20354006121243692No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24870.1968899775322173No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23820.1885773729319427No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23760.1881023669547842No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC23290.18438148680037558No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTGCCAGGCTAAGTAGTTGCTGTAAACAGTCTGA23290.18438148680037558No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23140.1831939718574792No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22850.18089810963454622No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22140.17527720557150345No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21400.16941879851988137No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20600.16308538549110077No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC20300.16071035560530803No Hit
CACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGACTTTGCAGTGTATTACTGTCAGCAGTATGCTACCACA20020.15849366104523482No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGA19950.15793948740521652No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19800.15675197246232014No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19270.152556086330753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19000.15041855943353954No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18750.1484393678620456No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18220.14424348173047843No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17640.13965175728461252No Hit
GTAGCATACTGCTGACAGTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTGTGTC17620.13949342195889297No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17490.13846424234171614No Hit
GAGTGAAGTGTGTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCA17330.13719755973596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16970.13434752387300875No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC16860.1334766795815514No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16830.13323917659297213No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC16680.13205166165007576No Hit
ACCCTCTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCT16500.13062664371860014No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16290.12896412279854522No Hit
GTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCAGTGGCCCTGTT16130.12769744019278909No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16040.1269849312270513No Hit
GTCTGGGACACACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGACT16010.126747428238472No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15710.12437239835267927No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15240.12065151819827066No Hit
GTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG15190.12025567988397187No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15090.11946400325537429No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTT15010.11883066195249624No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15000.11875149428963647No Hit
GCTGTAAACAGTCTGACTGGCCGTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA14630.11582229076382544No Hit
TCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCGGTGGCAGTGGGTCTGGGACACACTTCACT14400.11400143451805102No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14400.11400143451805102No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14170.1121805782722766No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13900.11004305137506314No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13380.10592633290635574No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGTTAC13270.1050554886148984No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13220.10465965030059961No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13010.1029971293805447No Hit
CTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAACAGGGCCA12890.1020471174262276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)