FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263142
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTACCACACCGAGGACGTTCGGCCAAGGGA63240.5006562999251074No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52680.41705524794520327No Hit
GTAATAGACAGCCGTGTCGTCAGTCCTCAGGCTGTTCATTTGCAGATACA43350.34319181849704944No Hit
CAGTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTGTGTCCCAGACCCACTGCCA39440.31223726231888416No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38850.3075663702101585No Hit
GTGTATTACTGTCAGCAGTATGCTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA36370.28793278982093856No Hit
CTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTAGCCTGGCACC36220.2867452748780422No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35660.2823118857578958No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35550.28144104146643845No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30660.24272805432801695No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30630.24249055133943767No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28460.22531116849887028No Hit
GTATGCTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28000.22166945600732144No Hit
GTGTGGTAGCATACTGCTGACAGTAATACACTGCAAAGTCCTCAGGCTCC27870.22064027639014458No Hit
GTCTATTACTGTGCGAGAGAGGGGGGGTCTGTTTATTATGCTTATAGCAGTGAGGGTTACCTTGATTATTGGGGC27080.21438603102422374No Hit
GTTTACAGCAACTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCTCC26510.20987347424121752No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCGGTGGCAGTGGGTCTGGGACACACTTCA26130.20686510305254674No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25910.2051234144696321No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGACTGTTTACAG25730.2036983965381564No Hit
CTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA24980.1977608218236746No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC23790.18833986994336346No Hit
GTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGA23660.1873106903261866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23440.18556900174327196No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22850.18089810963454622No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22600.1789189180630523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22370.17709806181727789No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22050.17456469660576562No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21660.17147715775423505No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21350.16902296020558258No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTGCCAGGCTAAGTAGTTGCTGTAAACAGTCTGA20810.16474790641115566No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20480.16213537353678367No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGA20470.16205620587392391No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20090.15904783468525313No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19770.15651446947374087No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19720.15611863115944208No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19530.1546144455651067No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19480.1542186072508079No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19260.15247691866789323No Hit
CACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGACTTTGCAGTGTATTACTGTCAGCAGTATGCTACCACA19240.1523185833421737No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC18840.14915187682778341No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18290.14479765537049674No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18190.14400597874189916No Hit
GAGTGAAGTGTGTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCA17540.13886008065601493No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17310.1370392244102405No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC17110.13545587115304533No Hit
GTAGCATACTGCTGACAGTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTGTGTC16900.13379335023299044No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16380.12967663176428304No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16360.12951829643856352No Hit
GTCTGGGACACACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGACT16330.12928079344998425No Hit
ACCCTCTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCT16080.1273016018784903No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC16070.12722243421563054No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15150.11993900923253284No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15140.11985984156967308No Hit
GTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCAGTGGCCCTGTT15000.11875149428963647No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15000.11875149428963647No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14890.11788064999817913No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14830.11740564402102059No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14440.11431810516949006No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14400.11400143451805102No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14390.11392226685519127No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14380.11384309919233151No Hit
GTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG14370.11376393152947174No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14240.1127347519122949No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTT13620.10782635681498992No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13430.10632217122065453No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGTTAC13180.10434297964916057No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13080.10355130302056302No Hit
GCTGTAAACAGTCTGACTGGCCGTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA13060.10339296769484349No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12790.10125544079763005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)