FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305788
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27710.21220902627379024No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25340.19405906624965155No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC25300.1937527378104256No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24970.19122552818681132No Hit
GTAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA22420.17169709018615578No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21620.16557052140163642No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20530.15722307143272873No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA19970.1529344732835652No Hit
CAGTAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA19800.15163257741685482No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19600.15010093522072496No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19280.1476503077069172No Hit
GTACAGCCCCACTGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA18920.1448933517538835No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18430.14114082837336536No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18320.14029842516549396No Hit
CCCCTGTGCTGGTCATCTATCAAGATGACAAGCGGCCCTCAGGGATCCCT17810.13639273756536283No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17750.13593324490652386No Hit
GTATTACTGTGCGAAAGATCGGGACTACGGTGGTAACCCCGGTGGTGATG17390.13317628895349015No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16920.12957692979258503No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16720.12804528759645517No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16490.12628389907090584No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16440.12590098852187337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16220.12421618210613054No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15960.12222504725116175No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15950.12214846514135526No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15590.11939150918832153No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15060.11533265736857744No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14710.1126522835253502No Hit
GACTATTACTGTCAGGCGTGGTACAGCCCCACTGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG14550.11142696976844634No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14490.11096747710960739No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14150.10836368537618664No Hit
CTATTACTGTCAGGCGTGGTACAGCCCCACTGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC13880.10629596841141135No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13590.10407508722702306No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13580.10399850511721656No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13530.10361559456818412No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13320.10200737026224778No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13140.10062889228573092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAG252.1022308E-457.4783558
GCGAATA252.1294277E-457.32905657
TTCGTCG200.00618402154.07739359
AGGGGGG3350.049.73222770
CTAACGC900.048.843433
GGCGAAT305.3028646E-447.65430556
GATCGGA306.3980476E-445.87337539
TCGTATG1900.043.56764640
GACCGAT3350.041.4327426
CGTGTAA350.00111898640.9493357
CCTAACG1000.040.5776182
TCTCGTA1950.040.49350438
AGACCGA3450.040.21020525
CGTATGC2100.039.50013441
ACCGATC3350.039.44036527
TATCGCG451.0530854E-438.008636
ATCTCGT2550.036.27195737
GTATCAA54950.036.127861
ACGAGAC3800.035.59303722
TATCTCG2850.034.80787336
Produced by FastQC (version 0.11.5)