FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305788
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41320.3164372777204263No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC30020.22989949363908996No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25370.19428881257907105No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24950.19107236396719837No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23540.18027428648448293No Hit
GTAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA21320.16327305810744164No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21030.16105217692305335No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18830.14420411276562503No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18000.1378477976516862No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA17910.13715855866342774No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17600.13478451325942647No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17490.13394211005155507No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG17460.1337123637221356No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17360.13294654262407068No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17160.13141490042794082No Hit
CAGTAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA17140.13126173620832784No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17110.13103198987890835No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16710.12796870548664868No Hit
GTATTACTGTGCGAAAGATCGGGACTACGGTGGTAACCCCGGTGGTGATG16610.12720288438858374No Hit
GTACAGCCCCACTGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA16570.12689655594935778No Hit
CCCCTGTGCTGGTCATCTATCAAGATGACAAGCGGCCCTCAGGGATCCCT16270.12459909265516302No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16240.12436934632574353No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16170.12383327155709808No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16140.12360352522767862No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16000.12253137569038773No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14100.10798077482715418No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13980.10706178950947627No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13970.10698520739966977No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG13810.1057598936427659No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13750.10530040098392696No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13090.10024598173669845No Hit
GACTATTACTGTCAGGCGTGGTACAGCCCCACTGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG13070.10009281751708547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGA5050.035.9952470
GTATCAA57050.035.066861
TATCAAC64950.030.8465442
ATCAACG66150.030.1268223
TCAACGC65950.030.0622334
AACGCAG66750.029.7502566
CAACGCA67050.029.6171465
CGCAGAG70800.027.953048
AGAGTAC70800.027.90533611
GCAGAGT71200.027.7969
CAGAGTA71350.027.64289510
TACCGCA500.00858269727.0180877
GTGAGCG3850.026.97973470
TGCGTAT659.290104E-426.21990633
GGTATCA36050.026.0597571
ATGGCAA6400.025.35936770
ATCTCCG5500.024.64221467
ACGCAGA81450.024.4224287
GTATAGG1251.5449768E-724.331191
GTCATAG6950.024.3117391
Produced by FastQC (version 0.11.5)