FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237634
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40360.32610610245032057No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACTCCCCAATTGTTGCTGCCTGCATATGAG37500.3029974936047329No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35570.28740322260054263No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35450.2864336306210075No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG31860.2574266705665811No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28040.22656132588471226No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27280.22042057668098972No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCA27000.21815819539540768No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26040.2104014595591265No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24460.1976351651619138No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT23940.19343359991726147No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACTCCCCAATTGTTG23940.19343359991726147No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAATTGGGGAGTATTCGG23820.19246400793772633No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23660.19117121863167946No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23020.18600006140749203No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22730.1836568807902821No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21860.17662733893865232No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC21810.17622334228051267No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA21360.17258737235725585No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21180.17113298438795316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20890.1687898037707432No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC20700.16725461646981257No Hit
GTGTTGGTACCAGGAGACAAAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA20140.16272985389864855No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19870.16054827194469448No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19540.15788189400097283No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT19490.15747789734283318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19350.15634670670004217No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19340.15626590736841425No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18840.15222594078701782No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18780.15174114479725023No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17990.14535799759864385No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17780.14366121163445736No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACACCA17750.14341881363957357No Hit
GTATTCGGTGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC17130.138409255078642No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16820.1359044757981762No Hit
GTGTATTACTGTGCGAGAGTTCCCCCTCTGAGGTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT16110.13016772325259326No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16040.12960212793119774No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15420.12459256937026617No Hit
ACCGAATACTCCCCAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAAT15220.1229765827377076No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15040.12152219476840487No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14990.12111819811026522No Hit
GGTTATAACTTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCAGTAAG14890.12031020479398595No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14890.12031020479398595No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATTGGGGAGTATTCGGTGGAGGGACCAAG14870.12014860613073008No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14720.11893661615631115No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14640.11829022150328773No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14470.11691663286561293No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14410.11643183687584535No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG14380.11618943888096157No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14000.11311906427910028No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13950.11271506762096065No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13790.11142227831491378No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13680.11053348566700655No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13590.10980629168235521No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACTCCCCAATTGTTGC13570.10964469301909935No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13290.10738231173351735No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13170.1064127197539822No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA13020.10520072977956325No Hit
GTCTCTGGTGGCTCCGTCAGCAGTGTTAGTTACTACTGGAGCTGGATCCG12650.10221115450932991No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT12530.10124156252979476No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12450.10059516787677132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.044.4259341
TCGTGAA6100.039.8431470
GTGGTAT3800.039.2077371
GTATCAA27700.033.9834751
GATATCG400.002850629233.8489122
TGGTATC4500.033.0913622
TATCAAC32150.028.8432432
ATCAACG32350.028.553383
GCGAACT605.969197E-428.2817428
TCAACGC32800.028.1582284
GTTTAAG1050.00713624328.05692970
CAACGCA33050.027.945235
AACGCAG33750.027.6663486
TGTTTCG550.00821708427.25996467
GACAGGT1504.3655746E-1127.0889841
CTCGTGA7100.026.95814169
CGCAGAG35700.026.344698
ACGCAGA35700.026.155167
AGAGTAC36650.025.38386211
GCAGAGT37500.024.8997129
Produced by FastQC (version 0.11.5)