FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705182
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24080.3414721305989092No Hit
GTAATAGACGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGACCAGGGTGG23040.3267241648255344No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACAAC21050.2985044995476345No Hit
GTCTATTACTGCGGAACATGGGAAAACAATTTCAGTTATATTGTTGTATTCGGTGGAGGGACCAAGCTGACCGTC20260.2873017178543979No Hit
CTATAGTCCGTCCCTCAAGGGTCGAGTGTCCATGTCCATGGACACGTCGA19730.27978592760450494No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19560.27737520243001096No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17620.24986457396813871No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15690.222495752869472No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14820.21015851227059115No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14700.2084568239121248No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACAACAATATAACTG14640.20760597973289163No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14340.20335175883672585No Hit
CACATGCACTGTGTCCGGTGACTCCATCAGCAGTGGCCACTACTGGGCCT14330.2032099514735203No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13880.1968286201292716No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13750.19498512440759974No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12740.1806625807238415No Hit
CTACTGGGCCTGGATCCGGCAGCCCCCCGGGAAGGGGCTGGAGTGGATTGGGAGTATTTTTGAAGGTGTGAGCAC12510.17740101137011438No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12250.17371401992677069No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12130.17201233156830437No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12120.17187052420509882No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGAAACTCCAACATTGGCAG11760.16676545912969987No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11320.16052593514865665No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11240.15939147624301242No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10880.15428641116761346No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10860.15400279644120243No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10800.15315195226196926No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10670.1513084565402974No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10650.15102484181388634No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10280.14577796937528184No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10210.14478531783284315No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10130.14365085892719895No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9970.1413819411159105No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9910.14053109693667734No Hit
CAATAATAGACGGCCGTGTCTGTGGCGGTGACCGACCTCAGGCTCAGAGA9840.13953844539423865No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9770.13854579385179996No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9640.13670229813012813No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9390.13315711404998995No Hit
ATAATAGACGGCCGTGTCTGTGGCGGTGACCGACCTCAGGCTCAGAGAAAACGTATTCCTCGACGTGTCCATGGA9370.1328734993235789No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG9300.13188084778114018No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9220.13074638887549597No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9080.1287610857906186No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9070.12861927842741308No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9050.128335663701002No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8990.12748481952176885No Hit
GTACCAACAGTTCCCAGGAACCGCCCCCAAACTCCTCATTTATGACACTAGTAAGCGACCCTCAGGTATTCCTGA8900.1262085532529191No Hit
CAGGTATTCCTGACCGATTCTCTGCCTCCAGCTCTGGCACGACAGCCACC8820.1250740943472749No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8780.12450686489445278No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8740.12393963544163067No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8580.12167071763034223No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8550.12124529554072565No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8480.12025264399828696No Hit
GTGTCCGGTGACTCCATCAGCAGTGGCCACTACTGGGCCTGGATCCGGCA8370.11869276300302617No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8320.11798372618699854No Hit
GGCCACTACTGGGCCTGGATCCGGCAGCCCCCCGGGAAGGGGCTGGAGTG8300.11770011146058747No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8230.11670745991814879No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8220.11656565255494326No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8160.1157148083757101No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8120.11514757892288799No Hit
CTATTATTGTGCGAGACCAGTCGGATATAATTATGGTTCGGGGTTCGACCCCTGGGGCCGGGGAGTCCTGGTCAC8040.11401312001724379No Hit
CCCCCAAACTCCTCATTTATGACACTAGTAAGCGACCCTCAGGTATTCCT8020.11372950529083273No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8010.11358769792762718No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7930.11245323902198297No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7860.11146058747954429No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7760.11004251384748902No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7580.10748998130978953No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG7470.10593010031452874No Hit
GTCCATGTCCATGGACACGTCGAGGAATACGTTTTCTCTGAGCCTGAGGTCGGTCACCGCCACAGACACGGCCGT7460.1057882929513232No Hit
CCCACTATAGTCCGTCCCTCAAGGGTCGAGTGTCCATGTCCATGGACACGTCGAGGAATACGTTTTCTCTGAGC7460.1057882929513232No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7440.10550467822491214No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACAACAATATAACTGA7430.10536287086170663No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7400.10493744877209003No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT7400.10493744877209003No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7380.10465383404567898No Hit
GGTCATCACCGGACTCCAGACTGGGGACGAGGCCGTCTATTACTGCGGAA7330.10394479722965135No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7280.10323576041362371No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT7220.10238491623439056No Hit
CTCCTGCTCTGGAAGAAACTCCAACATTGGCAGTAATTTTGTTTCTTGGTACCAACAGTTCCCAGGAACCGCCCC7110.10082503523912976No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT7070.10025780578630766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG150.002436228868.4136424
CGCAATA200.006734982652.92048657
GTGGTAT1350.048.1497651
TCGTATG1150.048.07630540
GGTATCA6550.047.0084081
TGGTATC1400.046.4136732
TCTCGTA1050.045.9480338
CGGGTAC306.5876823E-445.59939617
ACGAGAC1250.043.78162822
AATCTCG1100.043.77815236
GGCAATC1100.043.7095733
ATGCCGT1350.041.1247944
CGTATGC1350.041.00089341
ATCTCCG1350.040.5299210
CCGTCTT1400.039.80784647
CACGAGA1400.039.08519721
ATCTCGT1250.038.5632837
TATACAC1550.037.509183
GCAATCT1300.037.006134
GTATCAA16600.036.6848071
Produced by FastQC (version 0.11.5)