FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705182
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23520.33353091825939973No Hit
GTCTATTACTGCGGAACATGGGAAAACAATTTCAGTTATATTGTTGTATTCGGTGGAGGGACCAAGCTGACCGTC20720.29382485656185214No Hit
GTAATAGACGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGACCAGGGTGG18320.2597910893925256No Hit
CTATAGTCCGTCCCTCAAGGGTCGAGTGTCCATGTCCATGGACACGTCGA17740.25156626232660506No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17240.24447589416632867No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16770.2378109480956689No Hit
GTCTATGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGGAAGAAA16760.2376691407324634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16720.23710191127964125No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACAAC15670.22221213814306093No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14800.20987489754418007No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13860.19654500540286055No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13350.18931282987937867No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13210.18732752679450126No Hit
CACATGCACTGTGTCCGGTGACTCCATCAGCAGTGGCCACTACTGGGCCT12660.17952812181819727No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12550.1779682408229365No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12420.17612474510126463No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12400.17584113037485358No Hit
GGGTCATGGGCCCAGTCTATGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC12330.17484847883241492No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12080.17130329475227674No Hit
CTACTGGGCCTGGATCCGGCAGCCCCCCGGGAAGGGGCTGGAGTGGATTGGGAGTATTTTTGAAGGTGTGAGCAC11740.16648184440328878No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11740.16648184440328878No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11650.16520557813443906No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGAAACTCCAACATTGGCAG11540.16364569713917823No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11530.16350388977597274No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACAACAATATAACTG11500.16307846768635614No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11390.16151858669109534No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11180.15854063206377927No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10930.1549954479836411No Hit
GCACAGGGTCATGGGCCCAGTCTATGTTGACGCAGCCGCCCTCAGTGTCT10620.15059941972426977No Hit
CAATAATAGACGGCCGTGTCTGTGGCGGTGACCGACCTCAGGCTCAGAGA10590.1501739976346532No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10250.14535254728566527No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10040.1423745926583492No Hit
ATAATAGACGGCCGTGTCTGTGGCGGTGACCGACCTCAGGCTCAGAGAAAACGTATTCCTCGACGTGTCCATGGA9890.14024748221026628No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9770.13854579385179996No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9640.13670229813012813No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9410.13344072877640098No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9390.13315711404998995No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9250.13117181096511255No Hit
GTCATGGGCCCAGTCTATGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG9220.13074638887549597No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG9120.1293283152434407No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9070.12861927842741308No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9030.12805204897459094No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8840.12535770907368596No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8810.12493228698406936No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8690.12323059862560304No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8650.12266336917278094No Hit
GGCCACTACTGGGCCTGGATCCGGCAGCCCCCCGGGAAGGGGCTGGAGTG8610.12209613971995881No Hit
GTGTCCGGTGACTCCATCAGCAGTGGCCACTACTGGGCCTGGATCCGGCA8550.12124529554072565No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8530.1209616808143146No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT8380.1188345703662317No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8340.1182673409134096No Hit
CTATTATTGTGCGAGACCAGTCGGATATAATTATGGTTCGGGGTTCGACCCCTGGGGCCGGGGAGTCCTGGTCAC8190.11614023046532668No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8140.11543119364929905No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8130.11528938628609352No Hit
CAGGTATTCCTGACCGATTCTCTGCCTCCAGCTCTGGCACGACAGCCACC8130.11528938628609352No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8070.11443854210686036No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8000.11344589056442166No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7940.1125950463851885No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7910.11216962429557192No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7880.11174420220595534No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7860.11146058747954429No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7840.11117697275313324No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCATGGGCCCAGT7790.1104679359371056No Hit
GGTCATCACCGGACTCCAGACTGGGGACGAGGCCGTCTATTACTGCGGAA7720.10947528439466692No Hit
GTACCAACAGTTCCCAGGAACCGCCCCCAAACTCCTCATTTATGACACTAGTAAGCGACCCTCAGGTATTCCTGA7710.10933347703146137No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7600.10777359603620058No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG7570.107348173946584No Hit
ATGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC7540.10692275185696742No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7240.10266853096080161No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT7230.10252672359759608No Hit
GGACTATAGTGGGTGCTCACACCTTCAAAAATACTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGCCGGATC7220.10238491623439056No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7180.10181768678156845No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7140.10125045732874632No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7100.10068322787592424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA100.0090036133.8544370
CGTATTA150.002203748870.16579452
GTAGTTT601.0918286E-555.77268670
GTGGTAT1650.053.838291
TGGTATC2000.044.3945352
GTGCTAG704.8748916E-1043.9284931
GGTATCA6200.042.432721
CGAGTTC350.001173467140.55281460
GTATCAA16950.040.5161551
CCGAGTT350.001184998840.47226759
CGGTACC459.6092524E-538.6012345
TAACGGC451.0225429E-438.19603336
ATACGTA557.945684E-637.24094811
TATCAAC19300.035.3882262
ACGTACC601.4431522E-534.13511313
ATCAACG20500.033.476183
CAACGCA20800.033.3169175
TCAACGC20700.033.1480374
AACGCAG21650.032.3242236
CTAGAGA652.4939469E-531.5115684
Produced by FastQC (version 0.11.5)