FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194255
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35510.2973401827917823No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32680.273643401116177No Hit
GATTATTACTGTGGAGCATGGGAGGTCACCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAG32610.27305726164010197No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27470.2300178772540203No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC25970.21745774562384082No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGGTGACCTCCCATGCTCCACAGTAATAATCAG25530.21377344034565482No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGTCAGGGACG24680.20665603242188643No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24370.20406027188498269No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23170.1940121665808391No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT21190.17743279282900218No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19930.1668822822596514No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19930.1668822822596514No Hit
CCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC19800.1657937375183692No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC18860.15792272169679006No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18130.15181012430343604No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18060.151223984827361No Hit
CTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATA18060.151223984827361No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17910.14996797166434303No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17390.14561379269921415No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGGTGA17310.14494391901227124No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17290.1447764505905355No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC16140.13514701634073123No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG15820.13246752159295963No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15660.13112777421907382No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT15080.12627118998873774No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14970.12535011366919124No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14940.12509891103658766No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG14650.12267061892141963No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14650.12267061892141963No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA14520.1215820741801374No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13890.11630681889546202No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13380.112036374141201No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13250.11094782939991878No Hit
CCCCTGAGGGCCGCTCACTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATACCAGTAAA12990.10877073991735434No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12250.10257440831313246No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12130.10156959778271811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)