FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194255
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42130.3527722303863078No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31720.26560491687286214No Hit
GATTATTACTGTGGAGCATGGGAGGTCACCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAG30150.2524586457666076No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC29130.2439177562580856No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC27730.23219496673658474No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27310.22867812988013447No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23470.19652419290687498No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21990.18413152969843125No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT21330.17860507178115226No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGGTGACCTCCCATGCTCCACAGTAATAATCAG20040.16780335857919793No Hit
CCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC18370.15381974536426476No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18000.15072157956215382No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG17490.1464511348078928No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17230.14427404532532834No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC17200.14402284269272475No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17020.14251562689710323No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA16710.13991986636019946No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16620.1391662584623887No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16520.13832891635371006No Hit
CTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATA16030.13422594002118476No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15850.13271872422556322No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15430.12920188736911298No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG15330.12836454526043434No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15330.12836454526043434No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15050.12601998735613415No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGGTGA14510.12149833996926954No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14340.12007485838451586No Hit
ATAGGAATAGTGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCGTCCCTGA14200.11890257943236579No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14130.11831643995629075No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13260.11103156361078664No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12860.10768219517607212No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12700.10634244780218631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6500.043.3068471
TATACGT350.001561625638.26495
GTATCAA47050.034.187891
TATCAAC48850.031.743322
ATCAACG50650.030.8732533
TCAACGC51050.030.6300664
CAACGCA52400.029.9674765
AACGCAG52950.029.7194336
CGCAGAG55700.028.2521368
ACGCAGA56500.028.0298827
AGAGTAC57500.027.13253411
TCGGTTG500.00853591627.04817632
GCAGAGT58500.026.8998959
CAGAGTA58550.026.87467410
GGTATCA32050.025.8261431
GACAGGT3400.024.6406271
ACGGGAC2500.022.7771516
GAGTACG47750.022.22583812
AGTACGG47250.022.10768113
GACAGCC2650.021.4977861
Produced by FastQC (version 0.11.5)