FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793975
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30060.3786013413520577No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27330.34421738719732986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20310.25580150508517274No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19210.24194716458326776No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG19080.2403098334330426No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18460.23250102333196887No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18360.2312415378317957No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17780.22393652193079128No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15930.20063604017758746No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15530.19559809817689475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15000.18892282502597688No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14060.177083661324349No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13440.1692748512232753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13070.16461475487263452No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12950.16310337227242672No Hit
ACATATTACTGTCAACATTATGAAAGTCTCCCGCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGAA12920.16272552662237477No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12760.16071034982209767No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTGAGTA12600.1586951730218206No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12530.15781353317169936No Hit
AGTCAGGACATTGAGTACCGGTTAAATTGGTATCAGCACAAAGTAGGGAA12490.1573097389716301No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12160.1531534368210586No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11670.14698195787021No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11610.14622626657010612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCC11210.14118832456941338No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11110.1399288390692402No Hit
GTAATATGTCGCAACATCGTCAGGCTGCAGGTTGGTGATGGTGAAAGAAAAATCAGTCCCAGACCCACCTCCA10960.13803961081898045No Hit
ATATTACTGTCAACATTATGAAAGTCTCCCGCTCAGTTTCGGCGGAGGGA10730.13514279416858213No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10440.13149028621807993No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10370.1306086463679587No Hit
AAGTAGGGAAAGCCCCTAAACTCCTGATCTACAATGCATACAGATTGGAC10310.12985295506785477No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10250.12909726376775088No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10210.12859346956768158No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9790.12330363046695425No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9560.12040681381655592No Hit
GTCTGGGACTGATTTTTCTTTCACCATCACCAACCTGCAGCCTGACGATG9550.12028086526653863No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9470.11927327686640007No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTGAGTA9370.1180137913662269No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG9260.1166283573160364No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9120.11486507761579395No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9020.11360559211562077No Hit
GTATAGCCGCTGAGGCGGCCTCCAGAAGACGTGCAGGAGATTTTCACCGAGGACCCAGGCTTCTTCACCTCACCT8950.11272395226549954No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT8940.11259800371548223No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8880.11184231241537831No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8870.111716363865361No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8810.1109606725652571No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8790.11070877546522245No Hit
CAGTAATATGTCGCAACATCGTCAGGCTGCAGGTTGGTGATGGTGAAAGAAAAATCAGTCCCAGACCCACCTCCA8770.11045687836518782No Hit
GCACAAAGTAGGGAAAGCCCCTAAACTCCTGATCTACAATGCATACAGATTGGACACAGGGGTCCCGTCCAGGTT8710.10970118706508392No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8670.10919739286501463No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8660.10907144431499734No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8400.10579678201454706No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8390.10567083346452973No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8300.10453729651437388No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8290.10441134796435657No Hit
GTTCAGTGGAGGTGGGTCTGGGACTGATTTTTCTTTCACCATCACCAACC8240.10378160521426996No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8220.10352970811423534No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8180.10302591391416606No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8170.10289996536414873No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8160.10277401681413142No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC8130.10239617116407948No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8080.10176642841399287No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8030.1011366856639063No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCC8000.10075884001385434No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT7950.10012909726376776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATT307.5191892E-659.40666658
CCGATTC200.00640185853.60415659
TCGTATG1050.052.50683640
ACGTTAA200.00782128150.9572076
CTTATAC1250.048.9219971
CGTATGC1200.046.02286541
ATGCCGT1300.045.3730144
ATCTCGT1100.043.65434337
GCCGTCT1400.042.30352446
GTATGCC1500.039.19719742
GTGGTAT2450.038.826981
TCTAGTA350.001453799438.8245352
CGAGACA1700.037.98964323
TCTCCGA1650.037.05745311
GATCTCG1300.036.90308836
CACGAGA1800.035.86329321
TATACAC1800.035.8587723
TTATACA1850.034.8896182
GGGCGAA400.002625465634.4202339
GTATCAA24000.034.1151661
Produced by FastQC (version 0.11.5)