FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793975
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27460.345854718347555No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24930.3139897351931736No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20530.2585723731855537No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG19880.25038571743442806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18190.22910041248150131No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18080.2277149784313108No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17330.21826883718001197No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16230.20441449667810702No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15420.19421266412670424No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14280.17985452942473001No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14160.17834314682452218No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14080.17733555842438364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC14050.17695771277433167No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13220.1665039831228943No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12890.16234768097232283No Hit
AGTCAGGACATTGAGTACCGGTTAAATTGGTATCAGCACAAAGTAGGGAA12800.16121414402216697No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12680.15970276142195913No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12560.1581913788217513No Hit
ACATATTACTGTCAACATTATGAAAGTCTCCCGCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGAA12370.15579835637142225No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTGAGTA12350.15554645927138763No Hit
GTAATATGTCGCAACATCGTCAGGCTGCAGGTTGGTGATGGTGAAAGAAAAATCAGTCCCAGACCCACCTCCA12160.1531534368210586No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11920.15013067162064297No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11310.14244781006958657No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11010.13866935356906704No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCC10760.1355206398186341No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10440.13149028621807993No Hit
ATATTACTGTCAACATTATGAAAGTCTCCCGCTCAGTTTCGGCGGAGGGA10260.1292232123177682No Hit
GTCTGGGACTGATTTTTCTTTCACCATCACCAACCTGCAGCCTGACGATG9840.12393337321704083No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9810.12355552756698887No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9730.12254793916685035No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9650.12154035076671181No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9590.12078465946660788No Hit
GTATAGCCGCTGAGGCGGCCTCCAGAAGACGTGCAGGAGATTTTCACCGAGGACCCAGGCTTCTTCACCTCACCT9500.11965112251645202No Hit
AAGTAGGGAAAGCCCCTAAACTCCTGATCTACAATGCATACAGATTGGAC9490.11952517396643472No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9310.117258100066123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9230.11625051166598444No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG9180.11562076891589786No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9120.11486507761579395No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8960.11284990081551687No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8780.11058282691520514No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8610.10844170156491073No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8580.10806385591485877No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8570.10793790736484148No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8330.10491514216442582No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8310.1046632450643912No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTGAGTA8310.1046632450643912No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8270.10415945086432192No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT8240.10378160521426996No Hit
GCACAAAGTAGGGAAAGCCCCTAAACTCCTGATCTACAATGCATACAGATTGGACACAGGGGTCCCGTCCAGGTT8170.10289996536414873No Hit
GTTCAGTGGAGGTGGGTCTGGGACTGATTTTTCTTTCACCATCACCAACC8150.1026480682641141No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAAAGACCGTCCCTGTCCTTGGTACTGCAAACTACG8150.1026480682641141No Hit
CAGTAATATGTCGCAACATCGTCAGGCTGCAGGTTGGTGATGGTGAAAGAAAAATCAGTCCCAGACCCACCTCCA8070.10164047986397558No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7970.10038099436380239No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7960.10025504581378507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)