FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339921
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT56790.4238309571982229No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55430.41368110508007555No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52140.3891274187060282No Hit
ATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAATGTGGAAATCAAACGAACTGTGGCTGCACCAT51460.38405249264695457No Hit
GTTTTAGACACCTCCAATAATAAGAACTATTTAGCTTGGTACCAGCAGAA42710.31875013526916884No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38700.2888229977737493No Hit
GGTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAGTTCTTATTATTGGAGGTGTCTAAAACACT37250.27800146426543054No Hit
GTAATACACGGCCGTGTCGTCAGATGTCAGCCTGCTCAACTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT34640.2585227039504568No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33250.24814895803558565No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33080.24688022652081726No Hit
TTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAATGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT32660.24374571336668355No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31960.2385215247764607No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31090.23202860467146946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29840.2226996964746429No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29810.22247580267791908No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29190.2178486642122931No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29000.2164306701663755No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAATGTGGAAATCAAACGAA28120.20986311879580963No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC27950.20859438728104118No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGTCTTCTGGATACACCTTCACCGG26090.19471297188416334No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTCGGACGTT25250.1884439455758959No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25180.1879215267168736No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25050.18695132026440364No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24860.18553332621848603No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24480.18269733812665076No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24110.1799359813003901No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22280.16627845970023605No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT22090.16486046565431844No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21890.1633678403428262No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21490.16038258971984168No Hit
AACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAA21350.15933775200179712No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTAGA20770.15500913859846963No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20750.15485987606732038No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCA20630.15396430088042504No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19740.14732211824428457No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19710.1470982244475607No Hit
CTATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAATC19480.14538170533934464No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT19420.14493391774589695No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACATTGGTCCCTTGGCCGAACGTC19170.14306813610653166No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGC19120.1426949797786586No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19120.1426949797786586No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19090.14247108598193475No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18270.13635132220481655No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18180.13567964081464504No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18130.135306484486772No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17770.13261975892608593No Hit
GTCTAAAACACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16850.1257536824932216No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16620.12403716338500555No Hit
CTTTATACACTGGGTGCGACGGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATGGATCAACCCCATCAGTGGTGG16530.12336548199483402No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC16300.12164896288661795No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT16220.12105191276202104No Hit
CCTCCAATAATAAGAACTATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAACCTGCTCATTTACTGGG16040.11970854998167803No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15930.1188876060603573No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15720.11732034948329043No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15720.11732034948329043No Hit
CAGTAATACACGGCCGTGTCGTCAGATGTCAGCCTGCTCAACTCCATGTA15660.11687256188984275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15580.11627551176524586No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTT15270.11396194253243289No Hit
GTTATATCCTCTCTCGCACAGTAATACACGGCCGTGTCGTCAGATGTCAGCCTGCTCAACTCCATGTAGGCTGTG15210.1135141549389852No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15200.11343952367341059No Hit
ACTTTATACACTGGGTGCGACGGGCCCCTGGACAAGGGCTTGAGTGGCTG15090.11261857975208986No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15040.1122454234242168No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14890.11112595444059761No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14820.11060353558157533No Hit
GTGTATTACTGTGCGAGAGAGGATATAACAAGTCGTCCGAATGCTTTTGA14760.11015574798812766No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14700.10970796039467999No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14290.10664807850612089No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14240.10627492217824783No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14230.1062002909126732No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14200.10597639711594938No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14170.10575250331922553No Hit
GGATCTCTGGTGCCCACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14090.10515545319462864No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14080.10508082192905402No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13920.10388672167986022No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13790.10291651522739027No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13670.10202094004049492No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13620.10164778371262186No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13540.10105073358802497No Hit
CACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT13480.1006029459945773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT853.068999E-853.571270
TTGTGCG200.006893387552.61299552
ACCGATC2050.046.6388427
TATCGCG306.430599E-445.82627536
GGTATCA13800.044.0125271
AATGGTA4450.043.85443570
CGATCAG2200.043.49629229
TCGTATG1350.043.4929840
CGTATGC1300.042.5652741
ACGAGAC2400.041.229322
ATCTCGT1600.040.85704837
TATCTCG1700.040.4349536
TCAGTAT2400.039.95975532
GTGGTAT3950.039.73711
GTATCGC350.001379300639.2442535
ATGCCGT1600.039.02692844
CACGAGA2600.038.05639621
TGGTATC4250.037.726552
AGACCGA2600.036.7523425
GAGACCG2600.036.74959624
Produced by FastQC (version 0.11.5)