FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339921
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54720.40838228522427816No Hit
ATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAATGTGGAAATCAAACGAACTGTGGCTGCACCAT50470.37666399735506795No Hit
CTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT47860.35718523704009414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42950.3205412856429596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39610.29561444294103906No Hit
GGTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAGTTCTTATTATTGGAGGTGTCTAAAACACT38610.2881513163835778No Hit
GTTTTAGACACCTCCAATAATAAGAACTATTTAGCTTGGTACCAGCAGAA35430.26441857393085116No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC34960.2609109044488444No Hit
GTAATACACGGCCGTGTCGTCAGATGTCAGCCTGCTCAACTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT34460.25717934117011376No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32340.24135751286829596No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32260.24076046274369906No Hit
TTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAATGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT30940.23090913568785026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30620.22852093518946265No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30500.2276253600025673No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29980.22374453419268747No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT29110.21725161408769622No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAATGTGGAAATCAAACGAA28170.21023627512368268No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27790.2074002870318474No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27240.2032955674252437No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26090.19471297188416334No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25720.19195161505790267No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGTCTTCTGGATACACCTTCACCGG25250.1884439455758959No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24910.18590648254635908No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24550.18321975698567305No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23890.17829409345774863No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23360.1743386363822942No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23340.17418937385114494No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTCGGACGTT22970.1714280170248843No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22900.170905598165862No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22690.16933834158879515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22270.16620382843466144No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22230.165905303372363No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21520.16060648351656553No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACATTGGTCCCTTGGCCGAACGTC21130.15769586415915565No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19900.14851621849347835No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC19400.14478465521474773No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18990.14172477332618863No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18610.13888878523435338No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT18520.13821710384418184No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTAGA17800.1328436527228098No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17340.1294106145063776No Hit
AACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAA17090.1275448328670123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16790.12530589489977395No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16510.1232162194636848No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16510.1232162194636848No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16440.1226938006046625No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16380.12224601301121484No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16340.1219474879489164No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCA16000.11941002491937958No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT15980.11926076238823036No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15830.11814129340461117No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG15780.11776813707673811No Hit
GTTATATCCTCTCTCGCACAGTAATACACGGCCGTGTCGTCAGATGTCAGCCTGCTCAACTCCATGTAGGCTGTG15740.11746961201443966No Hit
CTATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAATC15510.11575309290622357No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGC15500.11567846164064896No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC14720.10985722292582922No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14550.1085884914110608No Hit
GTGTATAAAGTAGCCGGTGAAGGTGTATCCAGAAGACTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC14550.1085884914110608No Hit
CTTTATACACTGGGTGCGACGGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATGGATCAACCCCATCAGTGGTGG14440.10776754748974006No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14410.10754365369301623No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14400.10746902242744162No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14400.10746902242744162No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14290.10664807850612089No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14280.10657344724054627No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14040.10478229686675558No Hit
CAGTAATACACGGCCGTGTCGTCAGATGTCAGCCTGCTCAACTCCATGTA13960.10418524674215866No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13880.10358819661756179No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13860.10343893408641255No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTC13800.1029911464929649No Hit
ACTTTATACACTGGGTGCGACGGGCCCCTGGACAAGGGCTTGAGTGGCTG13700.10224483383721877No Hit
CCTCCAATAATAAGAACTATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAACCTGCTCATTTACTGGG13580.10134925865032342No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13570.1012746273847488No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13540.10105073358802497No Hit
GTCTAAAACACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA13500.1007522085257265No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC13430.10022978966670423No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13420.10015515840112962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)