FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193057
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCGACGTTCGGCCAAGGGA52380.4390402135019534No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41940.3515339166527668No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33350.2795340038237905No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30130.2525445137994245No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27680.23200903225914604No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26490.2220346555110108No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA25390.21281464339088577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25140.21071918609085735No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA24400.20451663248277327No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23670.19839789716669026No Hit
GTATGGTAGCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23330.19554807523865161No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22910.1920277069746039No Hit
GTAATAGACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGCAGATACA22070.18498697044650841No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22070.18498697044650841No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20440.17132458885032317No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20260.1698158595943027No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19610.16436767061422883No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCCACTTAGCCTGGTACC19200.16093112064218223No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18670.156488751166122No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG18490.15498002191010155No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17750.1487774683020174No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17570.14726873904599697No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17450.14626291954198334No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT16830.14106618543791286No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16430.1377134537578674No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16170.13553417816583785No Hit
GTGTTAGCAGCAGCCACTTAGCCTGGTACCAACGGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT16040.13444454036982306No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15940.1336063574498117No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15690.13151090014978328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15180.12723616725772532No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15180.12723616725772532No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15080.12639798433771396No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15060.12623034775371167No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14950.12530834654169917No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCCGTTGGTACCAGGCTAAGTGGCTGCTGCTAACACTCTGA14690.12312907094966963No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14280.11969252097762303No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13850.11608833442157415No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA13710.11491487833355823No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13430.11256796615752643No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC13350.11189741982151732No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13110.10988578081349006No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12840.10762268692945935No Hit
GTCTATTACTGTGCAAGAGGTTCCTTTATAGCAGCACCTGGTACTGTCAA12530.10502431987742414No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12410.1040185003734105No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12270.10284504428539458No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12270.10284504428539458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.042.624451
GTGGTAT3800.040.110651
TGGTATC3850.037.8079762
AACTGTG24450.036.12010270
GTATCAA30850.034.3652841
TATCAAC34750.030.5879652
ATCAACG35150.030.237343
CGTTATA450.005002130.17611527
TCTGGAG9450.030.14632470
GTAGAGT950.005155444629.9876670
TCAACGC35350.029.873514
CAACGCA36000.029.4269145
CGTGTTC6350.029.3939852
AGTTCGA604.912213E-429.2393450
AATGGTA4400.029.13573570
AACGCAG37050.028.9583576
CGATTAA1000.006623945728.48827670
GCGTTGT6700.027.99601262
CGTTGTC6750.027.89833363
CTCGACT6600.027.71513719
Produced by FastQC (version 0.11.5)