FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543148
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATAGTAGCTCGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGG96800.62728915178583No Hit
GTATAGTAGCTCGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT87570.5674763535318712No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77680.5033865837884636No Hit
CTACTATACTGCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTC74780.48459383027421865No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61570.3989895978869169No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA61030.39549025757736783No Hit
TCTATAGGTCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT56750.36775474549427534No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA52880.3426761399425072No Hit
CTCCTGTAGGGCCAGTCAGAGTATTGGCAGCAATTACTTAGCCTGGTATC52470.3400192334111829No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46400.3006840562279185No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45930.2976383341066443No Hit
GTGTATTACTGTCAGCAGTATAGTAGCTCGTGGACGTTCGGCCAAGGGAC41800.27087486099842656No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41630.2697732168269019No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39190.25396138283560615No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTAGACTGGAGTCTGAAGATT37200.24106566576893468No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35830.23218770979841208No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35790.23192849940511215No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35580.23056764484028752No Hit
GTAATACACTGCAAAATCTTCAGACTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34880.22603146295753873No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34590.22415218760611427No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTATTGGCAG34390.22285613563961462No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT32960.2135893640791421No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT32230.2088587744014184No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32100.20801634062319363No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31710.20548903928851933No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29140.18883477151899883No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA28130.1822897090881756No Hit
GACCTGGCCAGGCCCCCAGGCTCCTCATCTATAGGTCATCCACCAGGGCC27620.17898477657360148No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26780.17354135831430295No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26730.17321734532267805No Hit
ATAGTAGCTCGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGA25560.16563544131865512No Hit
GTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCTTGGCCGAACGTC25520.1653762309253552No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCC25280.1638209685655556No Hit
GCAGAGACCTGGCCAGGCCCCCAGGCTCCTCATCTATAGGTCATCCACCAGGGCCACTGGCATCCCAGACAGGTT25230.1634969555739307No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25160.1630433373856558No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA24380.15798873471630717No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24170.15662788015148255No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23890.15481340739838303No Hit
AGCTACTATACTGCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGTCTACTGATGGTGAGAGTGAAGT23700.1535821580302084No Hit
GACTTCACTCTCACCATCAGTAGACTGGAGTCTGAAGATTTTGCAGTGTA23670.15338775023523343No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC23240.15060123850725918No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22790.14768512158263497No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22570.14625946441948537No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22010.14263051891328635No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21880.14178808513506158No Hit
GAGTATTGGCAGCAATTACTTAGCCTGGTATCAGCAGAGACCTGGCCAGG21840.14152887474176165No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21830.14146407214343668No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA21210.13744631104728777No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20920.13556703569586326No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20830.13498381231093842No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGGAGAAATTGTGTTGACGCAGTCTC20670.1339469707377387No Hit
CCTGTAGGGCCAGTCAGAGTATTGGCAGCAATTACTTAGCCTGGTATCAGCAGAGACCTGGCCAGGCCCCCAGG20520.13297493176286396No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20410.13226210318128914No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAAATTGTGTTGACGCAGTC20140.13051243302651463No Hit
ACCTATAGATGAGGAGCCTGGGGGCCTGGCCAGGTCTCTGCTGATACCAGGCTAAGTAATTGCTGCCAATACT19110.12383776539904146No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19040.12338414721076656No Hit
CATCTATAGGTCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18400.11923678091796767No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17930.1161910587966935No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17460.11314533667541933No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17250.11178448211059472No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17160.11120125872566987No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17160.11120125872566987No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16730.10841474699769561No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16690.1081555366043957No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16510.10698908983454601No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16320.10575784046637134No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16240.10523941967977148No Hit
CTACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAGGACAGGGTGCCTGG16220.10510981448312152No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16160.10472099889317162No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15810.10245290795179725No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15630.10128646118194755No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15570.10089764559199767No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15510.10050883000204776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29450.038.365841
CGTATGC1550.037.93675241
TCGTATG1600.036.7308440
CGAGACG1650.035.3753323
ATGCCGT1700.034.6697744
GCGGCAT750.00266891234.30541670
ACGAGAC1800.032.42423622
TATCAAC35300.031.8028772
ATCAACG35300.031.8028773
CACGAGA1850.031.54688521
TCAACGC36100.031.0981034
CAACGCA36550.030.9970175
ATCTCCG1900.030.71172510
GTATGCC2050.030.39201542
AACGCAG37500.030.303316
ATCTCGT1950.030.061637
ACGCAGA39950.028.4375367
ACGTGTC500.00676225728.37096464
CGCAGAG40150.028.2103928
CCCACGA2200.028.08489619
Produced by FastQC (version 0.11.5)