FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543148
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATAGTAGCTCGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGG89350.5790112160337181No Hit
GTATAGTAGCTCGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT81860.5304740698883063No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76930.4985263889140899No Hit
CTACTATACTGCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTC70510.45692312078945113No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA60040.3890748003431946No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55220.357839947950553No Hit
CTCCTGTAGGGCCAGTCAGAGTATTGGCAGCAATTACTTAGCCTGGTATC53040.3437129815157069No Hit
TCTATAGGTCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT52640.34112087758270754No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA49010.3175975343907389No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48820.31636628502256425No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43990.2850666300315977No Hit
GTGTATTACTGTCAGCAGTATAGTAGCTCGTGGACGTTCGGCCAAGGGAC40880.2649130219525282No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40850.26471861415755327No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTAGACTGGAGTCTGAAGATT39520.2560998685803306No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39430.2555166451954058No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35720.23147488121683726No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35410.22946600066876283No Hit
GTAATACACTGCAAAATCTTCAGACTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33770.21883837454346572No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTATTGGCAG32370.20976601077796814No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31830.20626667046841912No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31760.20581305228014424No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT31560.2045170003136446No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29490.19110286246037322No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT29040.188186745535749No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA28880.18714990396254927No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27980.18131767011330086No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27430.1777535272054268No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27310.17697589602552705No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26350.17075484658632872No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26200.169782807611454No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25620.166024256908605No Hit
GACCTGGCCAGGCCCCCAGGCTCCTCATCTATAGGTCATCCACCAGGGCC25560.16563544131865512No Hit
GTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCTTGGCCGAACGTC25450.1649226127370803No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25310.16401537636053057No Hit
GAGTATTGGCAGCAATTACTTAGCCTGGTATCAGCAGAGACCTGGCCAGG24560.15915518148615687No Hit
ATAGTAGCTCGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGA24070.15597985416823273No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA23900.15487820999670804No Hit
GACTTCACTCTCACCATCAGTAGACTGGAGTCTGAAGATTTTGCAGTGTA23760.15397097362015827No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCC22730.1472963059926851No Hit
AGCTACTATACTGCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGTCTACTGATGGTGAGAGTGAAGT22440.14541703064126058No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21810.1413344669467867No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC21770.14107525655348677No Hit
GCAGAGACCTGGCCAGGCCCCCAGGCTCCTCATCTATAGGTCATCCACCAGGGCCACTGGCATCCCAGACAGGTT21710.14068644096353686No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20930.13563183829418823No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20550.1331693395578389No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20500.13284532656621398No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA20450.1325213135745891No Hit
CCTGTAGGGCCAGTCAGAGTATTGGCAGCAATTACTTAGCCTGGTATCAGCAGAGACCTGGCCAGGCCCCCAGG20350.13187328759133926No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20290.13148447200138938No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20110.13031802523153968No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20100.1302532226332147No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19780.12817953948681526No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAAATTGTGTTGACGCAGTC19660.12740190830691547No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19530.1265594745286907No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGGAGAAATTGTGTTGACGCAGTCTC19230.12461539657894123No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18570.12033842508949238No Hit
ACCTATAGATGAGGAGCCTGGGGGCCTGGCCAGGTCTCTGCTGATACCAGGCTAAGTAATTGCTGCCAATACT17640.11431178344526903No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17330.11230290289719458No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17130.11100685093069491No Hit
CATCTATAGGTCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16990.11009961455414516No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16760.10860915479267057No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16670.10802593140774573No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16620.10770191841612083No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16380.10614665605632125No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16290.1055634326713964No Hit
GGTCTCTGCTGATACCAGGCTAAGTAATTGCTGCCAATACTCTGACTGGCCCTACAGGAGAGGGTGGCTCTTTCC15910.10310093393504705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15900.10303613133672207No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15640.10135126378027254No Hit
CTACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAGGACAGGGTGCCTGG15520.10057363260037273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG607.383905E-444.55603470
GTATCAA32300.034.0857281
AATGGTA6300.032.88659770
ATACGTA1351.0913936E-1130.46250511
TATCAAC39400.027.7584742
CAACGCA40250.027.7568285
ATCAACG39800.027.7307283
TCAACGC40200.027.6208524
AACGCAG40750.027.5844526
TGTTAGA14350.027.4788170
GGGCGTA500.00798568827.4206963
AGACGTC500.00799200427.4162546
TGGATCG7250.026.7336270
GTCGGTT650.00952765426.44533369
ACGCAGA43300.025.880827
CGCAGAG43900.025.6051588
GAGTTTA4700.025.541141
AGAGTAC43900.025.37096411
GCAGAGT45700.024.671639
TCAAGCG2251.8493647E-623.76321870
Produced by FastQC (version 0.11.5)