FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522889
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23720.45363356276379885No Hit
GCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACGTAAAGCCACTGCCCATGTATAGCACA22500.43030165101962364No Hit
CTCATATATGACACAAACATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG22280.42609425709854293No Hit
ATACATGGGCAGTGGCTTTACGTTTTTCGGCGGAGGGACCAAGCTGACCGTCCCAGGTCAGCCCAAGGCTGCCCC18900.36145338685648387No Hit
GTATAGCACACAGAAATAGTCAGATTCATCTTCTGTCTGGGCCCCCGAGA17470.33410532636945894No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16780.32090940907152377No Hit
GACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACGTAAAGCCACTGCCCATGTATAGCACACAGAAATAGT16400.3136420922987479No Hit
TCCCAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15900.30407983338720074No Hit
GTGCTATACATGGGCAGTGGCTTTACGTTTTTCGGCGGAGGGACCAAGCTGACCGTCCCAGGTCAGCCCAAGG15760.3014024008919675No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT15250.29164889680218936No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15110.28897146430695614No Hit
ATATATGAGCGCGCGAGGAGTCTGGCCTGGGGTCTGCTGGTACCAACTGG14540.27807048914779237No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAAATCTGG14430.27596679218725195No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14260.2727156241573259No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTC14250.272524378979095No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13680.2616234038199312No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13530.258754726146467No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13050.24957495759138174No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCTCGGGGGCCCAGA13010.24880997687845796No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12750.2438376022444534No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12470.238482737253987No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11810.22586055549074469No Hit
GCCCATGTATAGCACACAGAAATAGTCAGATTCATCTTCTGTCTGGGCCCCCGAGATGGTGAGGGCAGCTTTGTT11710.22394810370843526No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11650.22280063263904962No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11580.22146191639143298No Hit
CCCCAGAAGAGCGAATGTTTGTGTCATATATGAGCGCGCGAGGAGTCTGG11250.21515082550981182No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT11250.21515082550981182No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11140.21304712854927146No Hit
CCCCAGGCCAGACTCCTCGCGCGCTCATATATGACACAAACATTCGCTCT11120.21266463819280956No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10990.21017845087580728No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10970.20979596051934538No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10410.19908623053841254No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10360.19813000464725786No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9510.1818741644976276No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9180.17556307361600645No Hit
GACTATTTCTGTGTGCTATACATGGGCAGTGGCTTTACGTTTTTCGGCGGAGGGACCAAGCTGACCGTCCCAGGT9180.17556307361600645No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAAATCTGGCTCAGTC9000.17212066040784949No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8970.17154692487315665No Hit
GTAGTAGAGACTGAGCCAGATTTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG8960.1713556796949257No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGG8800.1682957568432306No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8790.16810451166499965No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8710.1665745502391521No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8690.1661920598826902No Hit
CATATATGACACAAACATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC8470.16198466596160946No Hit
GAAATAGTCAGATTCATCTTCTGTCTGGGCCCCCGAGATGGTGAGGGCAG8370.1600722141793No Hit
CTCTACTACTAACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGACTCCTCGCGCGCTCATATATGACACAAA8110.15509983954529546No Hit
GTAATAAATGGCCGTGTCGGCGGCAGTCAGAGAGTTCACTTTCAGGGAGA8080.15452610401060263No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8040.15376112329767885No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT7920.15146618115890753No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7890.1508924456242147No Hit
CACTTGTGGCTTGAAATCTGGCTCAGTCTCTACTACTAACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGA7850.15012746491129092No Hit
GGCCTGGGGTCTGCTGGTACCAACTGGGGTAGTTAGTAGTAGAGACTGAG7610.14553758063374828No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7390.1413301867126675No Hit
CTACTACTAACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGACTCCT7300.13960898010858902No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG7270.13903524457389618No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7170.13712279279158673No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC7140.13654905725689392No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC7140.13654905725689392No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7020.13425411511812257No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6940.13272415369227503No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATT6910.13215041815758222No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAAAAA6890.13176792780112032No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG6760.12928174048411806No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6570.1256480820977301No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6520.12469185620657539No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6470.12373563031542067No Hit
GCTTGGTCCCTCCGCCGAAAAACGTAAAGCCACTGCCCATGTATAGCACACAGAAATAGTCAGATTCATCTT6470.12373563031542067No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC6340.1212494429984184No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6320.12086695264195652No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6300.12048446228549463No Hit
CCCATGTATAGCACACAGAAATAGTCAGATTCATCTTCTGTCTGGGCCCCCGAGATGGTGAGGGCAGCTTTGTTC6090.1164683135426448No Hit
TAGTAGTAGAGACTGAGCCAGATTTCAAGCCACAAGTGAGTGTGACTGTC6040.1155120876514901No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6030.11532084247325915No Hit
GTCACACTCACTTGTGGCTTGAAATCTGGCTCAGTCTCTACTACTAACTA5970.11417337140387349No Hit
CACCATCTCGGGGGCCCAGACAGAAGATGAATCTGACTATTTCTGTGTGC5920.11321714551271876No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5920.11321714551271876No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA5890.11264340997802592No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5860.1120696744433331No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACGTAAAGCCA5820.11130469373040933No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT5700.10900975159163799No Hit
GCCCTCACCATCTCGGGGGCCCAGACAGAAGATGAATCTGACTATTTCTG5660.1082447708787142No Hit
GAACAAAGCTGCCCTCACCATCTCGGGGGCCCAGACAGAAGATGAATCTGACTATTTCTGTGTGCTATACATGGG5650.1080535257004833No Hit
CTCCTCGCGCGCTCATATATGACACAAACATTCGCTCTTCTGGGGTCCCT5590.10690605463109762No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC5580.10671480945286668No Hit
ATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT5560.10633231909640478No Hit
GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACA5540.1059498287399429No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT5540.1059498287399429No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC5520.10556733838348101No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC5500.10518484802701912No Hit
GGACCAAGCTGACCGTCCCAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG5460.10441986731409535No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC5450.1042286221358644No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5390.10308115106647874No Hit
CAGTCACACTCACTTGTGGCTTGAAATCTGGCTCAGTCTCTACTACTAACTACCCCAGTTGGTACCAGCAGACCC5390.10308115106647874No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGG5330.10193367999709307No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG5320.10174243481886212No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGT5310.10155118964063117No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGA5310.10155118964063117No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGACTGTGGTGACC5300.10135994446240024No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA5280.10097745410593835No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA5270.1007862089277074No Hit
GGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGA5270.1007862089277074No Hit
GTCTGGGCCCCCGAGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC5260.10059496374947646No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC5260.10059496374947646No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT5250.10040371857124551No Hit
GTCTCTACTACTAACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGAC5230.10002122821478364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT100.007408738142.8664670
AGGGGGG1750.069.3922870
CGCGTAT150.002374541268.8547139
ACCTAAC1150.054.8878251
CAATGAT400.006410473553.57492470
CTTACCC253.094816E-453.1443563
CTAACGC1250.050.4871373
ACGTTAG401.0712938E-649.832371
TTACACC200.00854750549.8228344
CCTAACG1300.048.5453262
AATGGTA901.4613033E-647.62215470
TAACGCC1400.045.07784
CTCTTAT900.044.2954371
ACGCCAT1500.042.0726136
GACAGGT651.585795E-840.88811
AACGCCA1600.039.4430775
AGCATTG350.001623333737.9602555
CAGCTTA350.001623333737.9602556
TGTTTAC350.001627152837.94210414
AGCTTAT350.001627152837.9421047
Produced by FastQC (version 0.11.5)