FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522889
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30980.592477562159464No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23140.4425413424264041No Hit
CTCATATATGACACAAACATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG21540.41194211390945307No Hit
GCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACGTAAAGCCACTGCCCATGTATAGCACA19520.37331058790680244No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18720.3580109736483269No Hit
GTGCTATACATGGGCAGTGGCTTTACGTTTTTCGGCGGAGGGACCAAGCTGACCGTCCCAGGTCAGCCCAAGG17650.33754773957761586No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAAATCTGG17440.3335315908347661No Hit
ATACATGGGCAGTGGCTTTACGTTTTTCGGCGGAGGGACCAAGCTGACCGTCCCAGGTCAGCCCAAGGCTGCCCC16710.3195706928239072No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15100.2887802191287252No Hit
TCCCAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14210.27175939826617124No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT13980.2673607591668595No Hit
GACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACGTAAAGCCACTGCCCATGTATAGCACACAGAAATAGT13960.2669782688103976No Hit
ATATATGAGCGCGCGAGGAGTCTGGCCTGGGGTCTGCTGGTACCAACTGG13870.2652570622063191No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13700.2620058941763931No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13500.2581809906117742No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCTCGGGGGCCCAGA13470.2576072550770814No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13150.2514874093736912No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13060.24976620276961264No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12780.24441133777914625No Hit
GTATAGCACACAGAAATAGTCAGATTCATCTTCTGTCTGGGCCCCCGAGA12740.24364635706622248No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12670.24230764081860584No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTC12060.2306416849465183No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11790.22547806513428278No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT11750.224713084421359No Hit
CCCCAGGCCAGACTCCTCGCGCGCTCATATATGACACAAACATTCGCTCT11250.21515082550981182No Hit
CCCCAGAAGAGCGAATGTTTGTGTCATATATGAGCGCGCGAGGAGTCTGG10990.21017845087580728No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10870.20788350873703598No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10780.20616230213295747No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10760.20577981177649557No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10450.1998512112513363No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10240.1958350625084865No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10060.1923926493003295No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9430.18034420307178006No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9130.17460684772485174No Hit
GCCCATGTATAGCACACAGAAATAGTCAGATTCATCTTCTGTCTGGGCCCCCGAGATGGTGAGGGCAGCTTTGTT9120.17441560254662078No Hit
CTCTACTACTAACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGACTCCTCGCGCGCTCATATATGACACAAA9110.17422435736838984No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGG9100.1740331121901589No Hit
GACTATTTCTGTGTGCTATACATGGGCAGTGGCTTTACGTTTTTCGGCGGAGGGACCAAGCTGACCGTCCCAGGT9050.17307688629900417No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9000.17212066040784949No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8930.17078194416023287No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAAATCTGGCTCAGTC8850.1692519827343853No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8720.16676579541738304No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAAAAA8580.16408836292214984No Hit
GTAGTAGAGACTGAGCCAGATTTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG8450.16160217560514756No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8430.16121968524868566No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT8400.16064594971399285No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC8310.15892474310991433No Hit
CATATATGACACAAACATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC8270.15815976239699056No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7980.1526136522282932No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7960.1522311618718313No Hit
CACTTGTGGCTTGAAATCTGGCTCAGTCTCTACTACTAACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGA7920.15146618115890753No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7880.15070120044598376No Hit
GGCCTGGGGTCTGCTGGTACCAACTGGGGTAGTTAGTAGTAGAGACTGAG7720.14764127759428866No Hit
CTACTACTAACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGACTCCT7640.1461113161684411No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7600.14534633545551734No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7300.13960898010858902No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC7280.13922648975212712No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG7130.13635781207866296No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATT7060.13501909583104635No Hit
GTAATAAATGGCCGTGTCGGCGGCAGTCAGAGAGTTCACTTTCAGGGAGA6910.13215041815758222No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGT6760.12928174048411806No Hit
GTCACACTCACTTGTGGCTTGAAATCTGGCTCAGTCTCTACTACTAACTA6610.12641306281065387No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC6510.12450061102834444No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6200.11857201050318518No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6140.11742453943379952No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6140.11742453943379952No Hit
CACCATCTCGGGGGCCCAGACAGAAGATGAATCTGACTATTTCTGTGTGC6080.11627706836441386No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6060.11589457800795198No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG6020.11512959729502821No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA5920.11321714551271876No Hit
GAACAAAGCTGCCCTCACCATCTCGGGGGCCCAGACAGAAGATGAATCTGACTATTTCTGTGTGCTATACATGGG5920.11321714551271876No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC5910.11302590033448782No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG5890.11264340997802592No Hit
GCTTGGTCCCTCCGCCGAAAAACGTAAAGCCACTGCCCATGTATAGCACACAGAAATAGTCAGATTCATCTT5860.1120696744433331No Hit
GAAATAGTCAGATTCATCTTCTGTCTGGGCCCCCGAGATGGTGAGGGCAG5850.11187842926510215No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC5820.11130469373040933No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACGTAAAGCCA5820.11130469373040933No Hit
GTCTCTACTACTAACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGAC5790.11073095819571649No Hit
GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACA5770.1103484678392546No Hit
GGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGA5760.11015722266102365No Hit
GGACCAAGCTGACCGTCCCAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG5700.10900975159163799No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGA5680.1086272612351761No Hit
GCCCTCACCATCTCGGGGGCCCAGACAGAAGATGAATCTGACTATTTCTG5660.1082447708787142No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5630.10767103534402139No Hit
CTCCTCGCGCGCTCATATATGACACAAACATTCGCTCTTCTGGGGTCCCT5620.10747979016579046No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA5600.10709729980932857No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5540.1059498287399429No Hit
CAGTCACACTCACTTGTGGCTTGAAATCTGGCTCAGTCTCTACTACTAACTACCCCAGTTGGTACCAGCAGACCC5500.10518484802701912No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA5470.10461111249232628No Hit
ATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT5460.10441986731409535No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5420.10365488660117157No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGACTGTGGTGACC5390.10308115106647874No Hit
GGCCCAGACAGAAGATGAATCTGACTATTTCTGTGTGCTATACATGGGCA5380.10288990588824778No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGG5340.10212492517532401No Hit
AAGCTGCCCTCACCATCTCGGGGGCCCAGACAGAAGATGAATCTGACTAT5310.10155118964063117No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5230.10002122821478364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA150.00208006271.19205549
TTGTCTA150.00253940867.6989933
TCTATAC150.002721508766.526743
CCTAACG950.056.0439642
CGCAATG200.00587671854.7734757
ACGATGA400.006376628353.6466870
CTAACGC1000.053.2213943
ACCTAAC1050.050.716151
GGCCTAG200.00847945149.9237141
CTTGTAC200.00849871649.8950543
TAACGCC1150.046.270624
ACGCCAT1300.040.9277846
TAGGAAT2150.039.9231170
AAAGTCA750.001582640238.1487570
ATTATTC350.001611823338.015283
AACGCCA1550.034.326535
TCGCTAT400.003087544833.29841224
CTCGCTA400.003094797533.28247523
GTACGGA400.003094797533.2824751
GTGGCCG3250.033.0133470
Produced by FastQC (version 0.11.5)