FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500357
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT17320.3461528468673367No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16880.3373591255843328No Hit
AACATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG11760.2350321870184688No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11280.22543903652791908No Hit
CAATAATATATAGCCGTGTCTTCGTCTCTCAGACTGTTCATTTGCAGATA11250.2248394646222597No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10900.21784445905623384No Hit
GTATATGTTAGTACTTCTAGTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGACGGACCCAGTT9660.19306215362231366No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9250.1848680042449691No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9090.18167028741478583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8990.1796717143959213No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT8810.17607428296196517No Hit
ATAATATATAGCCGTGTCTTCGTCTCTCAGACTGTTCATTTGCAGATACA8710.17407570994310062No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8200.1638829875468915No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8140.1626838437355728No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7960.15908641230161666No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC7700.15389012245256886No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT7480.1494932618110669No Hit
ATATTATTGTGTGAGAGACTCAGACGCGATTTCTGCAGATATGGTGCATGGGGACTACTGGGGCCAGGGAACC7250.14489654386767847No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC7110.14209854164126812No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGTATGAACTGGGTCCGTCAGGCTCCAGGGAAGGGG6910.13810139560353907No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT6850.13690225179222035No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG6790.13570310798090165No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC6740.13470382147146936No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC6630.13250539115071838No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC6500.12990724622619448No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC6420.12830838781110288No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT6410.12810853050921642No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC6260.12511067098091963No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6210.12411138447148737No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA6150.12291224066016863No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT5850.11691652160357505No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT5810.11611709239602924No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC5810.11611709239602924No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG5780.11551752049036988No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT5740.11471809128282405No Hit
GTCTGAGAGACGAAGACACGGCTATATATTATTGTGTGAGAGACTCAGAC5450.10892222952811692No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA5450.10892222952811692No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA5420.10832265762245757No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC5350.10692365650925238No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCATGCA5240.10472522618850141No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG5230.10452536888661496No Hit
GTTTATTACTGCATGCAAGCTACACAACTTCGGGCGTTCGGCCAAGGGAC5220.1043255115847285No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC5210.10412565428284204No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5170.10332622507529624No Hit
ATATATTATTGTGTGAGAGACTCAGACGCGATTTCTGCAGATATGGTGCATGGGGACTACTGGGGCCAGGGAACC5110.10212708126397753No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG5100.10192722396209107No Hit
CTCTCAGACTGTTCATTTGCAGATACAGTGAGTTCTTGGCATTGTCTCTG5050.1009279374526588No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC5020.10032836554699945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG200.007529772451.4462837
CTCTTAT950.046.3890761
GTCGTAT605.680704E-945.92515639
TTATACA3950.045.4765932
GTGGTAT1200.045.199611
CTTATAC4150.044.1104661
TATACAC4200.042.7696533
CGTATGC4050.042.66198741
CTTGAAA4300.042.32254857
TCGTATG4200.041.8800140
ATGCCGT4250.041.71833444
TATGCCG4250.041.64147643
GCCGTCT4300.041.40305746
TGCCGTC4300.041.28827745
ATCTCGT3700.040.7862437
TGCTTGA4100.040.7299655
GCTTGAA4150.040.33646456
CCGTCTT4450.040.0610847
ACGAGAC4600.039.779422
GTATGCC4450.039.6846542
Produced by FastQC (version 0.11.5)