FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500357
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37380.7470665944515615No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17370.347152133376769No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12540.2506210565656121No Hit
AACATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG10450.20885088047134345No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9710.19406144013174592No Hit
CAATAATATATAGCCGTGTCTTCGTCTCTCAGACTGTTCATTTGCAGATA9340.18666671996194717No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9020.18027128630158068No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT8920.17827271328271613No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8880.17747328407517032No Hit
GTATATGTTAGTACTTCTAGTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGACGGACCCAGTT8540.17067813581103092No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8520.170278421207258No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8350.16688084707518833No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC8020.16028555611293538No Hit
ATAATATATAGCCGTGTCTTCGTCTCTCAGACTGTTCATTTGCAGATACA7570.151291977528045No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT7510.15009283371672624No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC7200.1438972573582462No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGTATGAACTGGGTCCGTCAGGCTCCAGGGAAGGGG7030.1404996832261765No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC7020.14029982592429005No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6950.1389008248110849No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT6800.13590296528278809No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6750.13490367877335582No Hit
ATATTATTGTGTGAGAGACTCAGACGCGATTTCTGCAGATATGGTGCATGGGGACTACTGGGGCCAGGGAACC6700.13390439226392356No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC6540.13070667543374032No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT6510.13010710352808094No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC6450.12890795971676222No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT6420.12830838781110288No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC6370.1273091013016706No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC6180.123511812565828No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT5800.11591723509414278No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT5800.11591723509414278No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5730.11451823398093762No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC5540.110720945245095No Hit
GTCTGAGAGACGAAGACACGGCTATATATTATTGTGTGAGAGACTCAGAC5460.10912208683000338No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC5360.10712351381113884No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5220.1043255115847285No Hit
GTTTATTACTGCATGCAAGCTACACAACTTCGGGCGTTCGGCCAAGGGAC5210.10412565428284204No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA5200.10392579698095561No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCATGCA5160.10312636777340979No Hit
GGACTAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC5100.10192722396209107No Hit
GGGTTCCCTGGCCCCAGTAGTCCCCATGCACCATATCTGCAGAAATCGCG5040.10072808015077234No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC5030.1005282228488859No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA5020.10032836554699945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG200.00794426150.7537278
GGTATCA2850.045.1369741
AATGGTA2200.039.8801870
GGTGTTA350.001427630338.9654333
GTATCAA8000.038.9306371
ATATGTA400.00224171735.54430455
TAACGGC400.002685135734.26034536
GCCTACT400.002843253833.8628923
GGTTATA400.002853026133.83922
TATCAAC9500.033.1267932
ATCAACG9450.032.5826383
TATGTAT450.003947784731.66806856
AAAGTAG450.00406714531.47598353
CGCCTTC450.00415111331.34485851
TCAACGC9750.031.233064
AACGCAG9900.031.1016086
CAACGCA9850.030.9159765
TGTTAGA2659.094947E-1230.56129870
AAATCAT450.005081032430.0762795
CGCTCGC604.7665113E-429.38580551
Produced by FastQC (version 0.11.5)