FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139065
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG27150.2383533863300163No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGCTGTGTCTCATAAGT21290.18690768305583966No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTGCTGGTTCTATATGAGCTGCAGTAATAAT20110.17654830935899182No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19190.1684715095275511No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTGCTGGTTCTATATGAGCTG19120.1678569704099415No Hit
GTCCTGGGCCCAGTCCGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG19100.16768138780491015No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18150.1593412140659225No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17990.15793655322567193No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16750.14705043171373014No Hit
GATTATTACTGCAGCTCATATAGAACCAGCAACACTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGT16050.14090504053763392No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC15770.13844688406719546No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15450.13563756238669436No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14950.13124799726091135No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14840.13028229293323912No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13920.1222054931017984No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13730.12053745835400088No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13620.11957175402632861No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13490.11843046709362504No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13180.11570893671563959No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13080.11483102369048298No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12920.11342636285023243No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12600.11061704116973133No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCAGCTCATAT12380.1086856325143868No Hit
CTCCTGCACTGGAACCAGCACTGACGTTGGTGGTTATGACTATGTCTCCTGGTATCAGCAGCACCCAGGCAAAGT11610.101925702220681No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11590.10175011961564967No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11540.10131116310307137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC200.00554002555.60099865
CGAGCAT605.8674085E-446.68163770
CCTAACG551.7805723E-742.8499762
CTAACGC603.5249286E-739.2791443
GTATCAA34450.035.1826441
TACGTAT1001.7060958E-435.0112370
CGTATGC1154.5474735E-1133.06184441
TCTCCGA2600.032.3700811
TCGTATG751.677643E-632.17912340
TATACAC3150.032.064613
GTGGTAT3700.031.8479541
TGCCGAT5450.030.83557970
CCCACGA2750.030.60443919
TATCAAC39600.030.4370842
TCAACGC39900.030.1238564
ATCAACG40050.030.0950983
CTTATAC2950.029.6733471
CAACGCA40500.029.5944445
ATCTCCG2850.029.53059810
CACGAGA2750.029.3828421
Produced by FastQC (version 0.11.5)