FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501277
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53820.3584948014257196No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44470.2962144893980258No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA40410.2691708458865352No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38470.2562485137652812No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT37330.2486549783950597No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAG36110.24052856334973494No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33960.22620742208133474No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32380.21568304849804532No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30060.20022953792005072No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTCGCCCTGTT29730.19803140926024976No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCTACTTAGCCTGGTACCAACAGAAAGAAGGCCAGGCTCCCAGGCT28970.19296905234676878No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27290.18177857916960027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27290.18177857916960027No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACC27260.18157874929143655No Hit
ATCCAACAGGGCGACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG27150.18084603973816957No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26460.17624995254040393No Hit
TCTTTGATGTATCCAACAGGGCGACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT26120.17398521392121508No Hit
GCGTAGCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG25780.1717204753020262No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24870.1656589689977266No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC24750.1648596494850717No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24550.16352745029731355No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24260.16159576147506421No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23350.15553425517076463No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAGCTACTTAGCCTGGTA23290.15513459541443717No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23260.15493476553627344No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23120.15400222610484274No Hit
GTTTATTACTGTCAGCAGCGTAGCACCTTCGGCCAAGGGACACGACTGGA22580.1504052882978957No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22160.1476076700036036No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA20930.13941464499889095No Hit
GTATCCAACAGGGCGACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC20460.13628397690765928No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19880.13242059926316063No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19430.1294231510907048No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19220.12802434194355872No Hit
GTTGGATACATCAAAGATGAGGAGCCTGGGAGCCTGGCCTTCTTTCTGTT19000.12655892283702475No Hit
GTCAGAGTGTTATCAGCTACTTAGCCTGGTACCAACAGAAAGAAGGCCAG18930.1260926531213094No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18920.12602604316192148No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGCTACGCTGCTGACAGTAATAAACTG18890.12582621328375776No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18670.1243607941772238No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA18300.1218962256798712No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT18240.12149656592354377No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17760.11829928787292418No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17760.11829928787292418No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17380.1157681094161837No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17030.11343676083760693No Hit
CATCAAAGATGAGGAGCCTGGGAGCCTGGCCTTCTTTCTGTTGGTACCAGGCTAAGTAGCTGATAACACTCTGA16930.11277066124372784No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16570.11037270270576316No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16310.10864084376167756No Hit
GAGTGTTATCAGCTACTTAGCCTGGTACCAACAGAAAGAAGGCCAGGCTCCCAGGCTCCTCATCTTTGATGTATC16100.10724203461453151No Hit
CTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACACAGTC15900.10590983542677335No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15890.10584322546738542No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15740.10484407607656682No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15670.10437780636085148No Hit
CTCCCAGGCTCCTCATCTTTGATGTATCCAACAGGGCGACTGGCATCCCA15560.10364509680758446No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15220.10138035818839561No Hit
CTGTTGGATACATCAAAGATGAGGAGCCTGGGAGCCTGGCCTTCTTTCTG15110.10064764863512864No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15040.10018137891941326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC200.0080485350.5915267
CGTATGC2200.043.92544641
GGTATCA16650.041.5265731
TCGTATG2350.039.56944340
GACTAGG2650.036.99209626
ATCTCGT2100.035.83508737
ACGAGAC2750.035.59464622
ATGCCGT2850.035.35816644
CCGTCTT2800.034.96693847
GTATCAA39800.033.8124281
GCCGTCT2900.033.7006646
TAGGCAT2950.033.29799329
CGAGACT2950.033.18919423
CACGAGA2850.033.1580621
GTGGTAT3800.032.840111
ACTAGGC3000.032.69381327
GTATGCC3350.032.01692642
CGTCTTC3200.031.7545848
CTTATAC3300.031.6835821
CCCACGA3050.030.9703419
Produced by FastQC (version 0.11.5)