FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501277
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51690.3443068800760952No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36910.24585736010076753No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36530.24332618164402703No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA35150.23413400724849576No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT33540.22340980378704264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33410.22254387431499983No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC32520.21661558792947605No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30270.2016283470671968No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAG29830.19869750885412887No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29090.19376837185942367No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTCGCCCTGTT27330.18204501900715192No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCTACTTAGCCTGGTACCAACAGAAAGAAGGCCAGGCTCCCAGGCT27220.18131230945388493No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25760.1715872553832504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25680.17105437570814713No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACC25480.16972217652038896No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25250.1681901474544671No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24690.16445998972874426No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23800.15853170334322048No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAGCTACTTAGCCTGGTA23530.15673323443974696No Hit
ATCCAACAGGGCGACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG23500.15653340456158324No Hit
TCTTTGATGTATCCAACAGGGCGACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT22600.15053850821667153No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22590.15047189825728363No Hit
GCGTAGCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG22330.14874003931319804No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21970.14634208077523336No Hit
GTTTATTACTGTCAGCAGCGTAGCACCTTCGGCCAAGGGACACGACTGGA21300.14187921349624352No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21050.14021396451154583No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20920.13934803503950305No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20540.13681685658276252No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA20220.1346853378823495No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19370.12902349133437735No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19250.12822417182172244No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19230.12809095190294664No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGCTACGCTGCTGACAGTAATAAACTG18870.12569299336498196No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18520.12336164478640517No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18450.12289537507068982No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18150.1208970762890526No Hit
GTATCCAACAGGGCGACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC17840.11883216754802743No Hit
GTTGGATACATCAAAGATGAGGAGCCTGGGAGCCTGGCCTTCTTTCTGTT17670.117699798238433No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17010.1133035409188311No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16910.11263744132495201No Hit
GTCAGAGTGTTATCAGCTACTTAGCCTGGTACCAACAGAAAGAAGGCCAG16850.11223778156862457No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16590.11050592262453898No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16590.11050592262453898No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC15750.10491068603595473No Hit
GTCGTGTCCCTTGGCCGAAGGTGCTACGCTGCTGACAGTAATAAACTGCA15720.104710856157791No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA15580.1037783167263603No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15570.10371170676697239No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15570.10371170676697239No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15330.1021130677416626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.042.070111
CTAACGC603.5242374E-739.281533
CCTAACG656.5864333E-736.2683332
GTATCAA40500.034.779611
GTGGTAT4300.032.9142721
GTATAGA752.0061689E-631.4514161
ATCAACG45200.031.137193
TCAACGC45000.031.123864
TATCAAC45450.030.9731452
AACGCAG46050.030.7797536
CAACGCA46100.030.3812085
TGTTAGA16300.029.34261770
ACGCAGA48600.029.2340417
CGCAGAG48700.029.174018
AGAGTAC49750.028.82609411
GCAGAGT50300.028.3129449
GTACGAA500.00752436827.7570243
TGGTATC5000.027.6157442
TACGTAT1050.007723821327.6066270
ACGGGTA2550.025.09906816
Produced by FastQC (version 0.11.5)