FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756291
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGA34610.4576280823122316No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23950.31667704626922705No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23200.30676022853637025No Hit
CAATAATACACTGCGAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21610.2857365749427139No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18100.23932586795294403No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA17560.23218575918528714No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16010.2116910025373831No Hit
GTGTATTATTGTCAGCAGTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15730.20798872391711654No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15700.2075920512078023No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14960.19780745771138358No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14460.19119624588947906No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14180.1874939672692125No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13480.17823827071854617No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13150.17387487091608916No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATT13140.17374264667965109No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12490.1651480713111752No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12460.16475139860186092No Hit
ATAATACACTGCGAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12050.15933020490789923No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11790.15589237476050885No Hit
GTCCCAGACCCACTGCCACTGATCCTGTCTGGGAGACCAGGGGACCTGGT11490.15192564766736613No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11300.1494133871750424No Hit
GAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC11260.14888449022929007No Hit
GTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11210.1482233690470996No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT11130.14716557515559486No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10220.13513316963972863No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCT10160.13433982422110008No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9860.13037309712795736No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCCGGGTGAG9700.1282575093449479No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGGTCCTTAGGCTGGTT9570.12653859427125275No Hit
GTCAGAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG9550.12627414579837654No Hit
GACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATTTCGCAGTGTATTATTGTCAGCAGTATGGCAGCTCA9530.12600969732550038No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9360.12376188530605282No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8880.11741512195702448No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGATCCTGTCTGGGAGACCA8870.1172828977205864No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8860.11715067348414829No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC8710.11516730993757693No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8670.11463841299182458No Hit
GTCTCCGGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT8620.11397729180963412No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8400.11106835860799613No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8390.11093613437155804No Hit
GTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCA8360.11053946166224375No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8230.10882054658854859No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC7810.10326712865814879No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7810.10326712865814879No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7800.1031349044217107No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7610.10062264392938698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC353.6536403E-758.241283
CCTAACG452.0681218E-645.2987752
GGTATCA9500.044.7027361
TCGTATG2100.042.68981640
CGAGACG2100.042.09654623
CGTATGC2150.041.78957741
ATCTCGT2000.041.1773237
CCGTCTT2150.040.50923547
ATGCCGT2150.040.36417844
ACGAGAC2250.039.27971622
GTATCAA25900.039.0898671
CTTATAC2550.038.6371881
GGAGCTA2400.036.944529
TATACAC2600.036.587473
ATCTCCG2600.036.58505210
TTATACA2700.036.4906772
CACGAGA2450.036.06843621
ATCAACG29100.034.4410743
TCAACGC29100.034.3243264
GTTAGCC400.002798830633.974083
Produced by FastQC (version 0.11.5)