FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153564
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC74330.34514878591952686No Hit
GCCTTGGGCTGACCCAGGACGGTCACCCTGGTCCCTCCGCCAAAAAGGACGATGGTATATCTTGTATATGAGTTG64240.2982962196619186No Hit
ATATTATTGTGCGAAGAGACGTCAGTGGCTGGCTGTCGAGACACCCTGGC63460.29467431662119165No Hit
CAATAATATAGGGCCGTGTCGTCGGCCGTCAGGCTGCCCATTTGCAGCGA57540.2671850012351618No Hit
CTCCTGTGCAGCCTCTGGCTTCACTTTCAGTCACTACGGCATGATTTGGGTCCGCCAGGCTCCGGGGAAGGGG49490.22980510446868538No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49010.2275762410590073No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45300.21034898428837034No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA44780.20793438226121905No Hit
GTCCTGGGCCCAGTCTCCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC44610.20714499313695808No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG43390.2014799653040263No Hit
GCCCTATATTATTGTGCGAAGAGACGTCAGTGGCTGGCTGTCGAGACACC42450.19711510779340663No Hit
CTCTTGTACTGGAAGCAGCAGTGACATTGGGGCTTATAATTATGTCTCCTGGTATCAACATCATCCAGGCTCAGC41770.1939575512963627No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCCTGGTCCCTC40250.18689948383238203No Hit
GGGCACAGGGTCCTGGGCCCAGTCTCCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGACGATCAC39150.18179167185186973No Hit
ATATAGGGCCGTGTCGTCGGCCGTCAGGCTGCCCATTTGCAGCGACAGCA38700.17970211240529652No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT38560.1790520272441404No Hit
TTTCTGGACTCCAGGCTGAAGACGAGGCTGATTATTACTGCAACTCATAT37140.1724583063238427No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG33840.1571348703823058No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33420.15518461489883745No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA33410.15513818024446918No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC33200.154163052502735No Hit
GATTATTACTGCAACTCATATACAAGATATACCATCGTCCTTTTTGGCGGAGGGACCAGGGTGACCGTCCTGGGT32250.14975176033774712No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31980.14849802466980316No Hit
ATAATATAGGGCCGTGTCGTCGGCCGTCAGGCTGCCCATTTGCAGCGACA31310.14538690282712752No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31150.14464394835723482No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTT30970.1438081245786055No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC30860.14329734338055428No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30390.14111491462524448No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30080.13967544033982737No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30020.1393968324136176No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29640.13763231554762245No Hit
GGTATATCTTGTATATGAGTTGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAAATGGTCAGGGAGGC29570.1373072729670444No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG29180.13549632144668095No Hit
TCCTCACCCTCCTCACGCAGGGCACAGGGTCCTGGGCCCAGTCTCCCCTGACTCAGCCTGCCTCCGTGTCTGGGT29100.1351248442117346No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29100.1351248442117346No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28610.1328495461476882No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT28430.13201372236905892No Hit
ATGGTATATCTTGTATATGAGTTGCAGTAATAATCAGCCTCGTCTTCAGC27840.1292740777613296No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27810.12913477379822472No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC27790.12904190448948813No Hit
CCCTGGTCCCTCCGCCAAAAAGGACGATGGTATATCTTGTATATGAGTTGCAGTAATAATCAGCCTCGTCTTCAG27770.12894903518075154No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAAATGGTCAGGGAGG27730.12876329656327834No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG27690.1285775579458052No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG27660.1284382539827003No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27530.1278346034759125No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26920.12500208955944656No Hit
CTCCTGGACAGACGATCACCATCTCTTGTACTGGAAGCAGCAGTGACATT26730.12411983112644899No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26730.12411983112644899No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG26400.12258748753229531No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26170.12151949048182455No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC26100.12119444790124649No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC25920.1203586241226172No Hit
GTCCAGAAATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCGGAGAAG25730.11947636568961963No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25650.11910488845467329No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25590.11882628052846353No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG25200.11701532900810006No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25140.1167367210818903No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24450.11353272993047804No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT24380.11320768734989997No Hit
GGCCTGGGCTCTGCTCTTCCTCACCCTCCTCACGCAGGGCACAGGGTCCTGGGCCCAGTCTCCCCTGACTCAGC23820.11060734670527554No Hit
GTAGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23740.11023586947032918No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT23470.10898213380238525No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTTCTTGT23460.10893569914801696No Hit
GGGTAGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23400.1086570912218072No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGACTGCCGCGG23400.1086570912218072No Hit
ACATCATCCAGGCTCAGCCCCCAAACTCATTATTTATGATGTCGTTAATCGGCCCTCAGGGATTTCTGATCGCTT22730.10554596937913154No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22430.10415292974808271No Hit
GTATCAACATCATCCAGGCTCAGCCCCCAAACTCATTATTTATGATGTCG22410.10406006043934612No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTAGAGAACCTATGAACATTC22390.10396719113060952No Hit
CCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGACGATCAC22300.1035492792412949No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT22290.10350284458692662No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22290.10350284458692662No Hit
ATATACCATCGTCCTTTTTGGCGGAGGGACCAGGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA22180.10299206338887537No Hit
GTGTGGGGAGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTGCAGCCGGGGGGGTCTCTGACACTCTCCTGTGCAGC22120.10271345546266561No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21980.10206337030150948No Hit
GAGTTTGGGCTGAGCTGCCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGGGGAGCAGCTGTTGGAGT21940.10187763168403632No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21740.10094893859667045No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC21550.10006668016367287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5650.046.4086170
GTCGTAT306.32311E-445.98458539
CGCTAAT3800.045.1329432
CGGCAGT24600.044.50086270
GCTAATC3500.043.14947533
CGTATGC4100.042.99341
TCGTATG4100.042.95363640
TCGCTAA4000.042.8363331
TCTCGTA3750.041.34250338
CTCGCTA4300.039.8180630
CTTATAC4650.039.6678661
TATGCCG4550.039.58429343
CTAATCT3850.039.25427634
AATCTCG4050.039.0676236
CACTCGC4400.038.8705728
ACACTCG4500.038.74529327
ACTCGCT4450.038.45170629
GTGGTAT3850.038.150981
ATCTCGT4100.037.77963637
TGTCGTA557.468243E-637.5840938
Produced by FastQC (version 0.11.5)