FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153564
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC69490.32267441320527274No Hit
GCCTTGGGCTGACCCAGGACGGTCACCCTGGTCCCTCCGCCAAAAAGGACGATGGTATATCTTGTATATGAGTTG60920.28287991441164506No Hit
ATATTATTGTGCGAAGAGACGTCAGTGGCTGGCTGTCGAGACACCCTGGC59570.2766112360719254No Hit
GTCCTGGGCCCAGTCTCCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC51410.23872055810739778No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50780.23579517488219529No Hit
CTCCTGTGCAGCCTCTGGCTTCACTTTCAGTCACTACGGCATGATTTGGGTCCGCCAGGCTCCGGGGAAGGGG50430.23416996197930498No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48900.22706545986095608No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46050.21383158336599237No Hit
GGGCACAGGGTCCTGGGCCCAGTCTCCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGACGATCAC45980.2135065407854143No Hit
CAATAATATAGGGCCGTGTCGTCGGCCGTCAGGCTGCCCATTTGCAGCGA45170.20974533378158253No Hit
GCCCTATATTATTGTGCGAAGAGACGTCAGTGGCTGGCTGTCGAGACACC42060.19530415627304318No Hit
CTCTTGTACTGGAAGCAGCAGTGACATTGGGGCTTATAATTATGTCTCCTGGTATCAACATCATCCAGGCTCAGC41230.19145007996047483No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC39420.18304540751981366No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCCTGGTCCCTC36550.16971866171611338No Hit
TTTCTGGACTCCAGGCTGAAGACGAGGCTGATTATTACTGCAACTCATAT35570.1651680655880206No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA35410.1644251111181279No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG34130.15848147535898632No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA33390.15504531093573257No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT32730.1519806237474252No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32490.15086619204258614No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32470.15077332273384958No Hit
GATTATTACTGCAACTCATATACAAGATATACCATCGTCCTTTTTGGCGGAGGGACCAGGGTGACCGTCCTGGGT32030.14873019794164466No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG31210.14492255628344455No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC31020.14404029785044697No Hit
ATATAGGGCCGTGTCGTCGGCCGTCAGGCTGCCCATTTGCAGCGACAGCA30950.14371525526986892No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTT30200.1402326561922469No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29520.13707509969520293No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29430.13665718780588829No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28440.13206015702342722No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28120.13057424808364182No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC28060.13029564015743206No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28050.13024920550306376No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27830.1292276431069613No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27770.12894903518075154No Hit
GGTATATCTTGTATATGAGTTGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAAATGGTCAGGGAGGC27490.12764886485843932No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG27470.12755599554970273No Hit
CCCTGGTCCCTCCGCCAAAAAGGACGATGGTATATCTTGTATATGAGTTGCAGTAATAATCAGCCTCGTCTTCAG27040.12555930541186608No Hit
TCCTCACCCTCCTCACGCAGGGCACAGGGTCCTGGGCCCAGTCTCCCCTGACTCAGCCTGCCTCCGTGTCTGGGT26890.12486278559634169No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26750.12421270043518559No Hit
ATGGTATATCTTGTATATGAGTTGCAGTAATAATCAGCCTCGTCTTCAGC26550.1232840073478197No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG26510.12309826873034653No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26490.12300539942160994No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT26410.12263392218666358No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25690.11929062707214644No Hit
GGCCTGGGCTCTGCTCTTCCTCACCCTCCTCACGCAGGGCACAGGGTCCTGGGCCCAGTCTCCCCTGACTCAGC25690.11929062707214644No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG25590.11882628052846353No Hit
GTGTGGGGAGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTGCAGCCGGGGGGGTCTCTGACACTCTCCTGTGCAGC25370.11780471813236107No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25290.1174332408974147No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAAATGGTCAGGGAGG25260.11729393693430983No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG25070.11641167850131225No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24310.11288264476932192No Hit
CCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGACGATCAC24150.11213969029942922No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG23970.11130386652079993No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23860.11079308532274872No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC23840.11070021601401211No Hit
GTCCAGAAATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCGGAGAAG23610.10963221896354138No Hit
CAGTGGTGCTGCCACATTCTACGCAGACTCTGTGAAAGGCCGCTTCACCG23560.1094000456916999No Hit
GAGTTTGGGCTGAGCTGCCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGGGGAGCAGCTGTTGGAGT23500.10912143776549013No Hit
GTAGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23490.10907500311112184No Hit
CTCCTGGACAGACGATCACCATCTCTTGTACTGGAAGCAGCAGTGACATT23220.10782126744317791No Hit
ATAATATAGGGCCGTGTCGTCGGCCGTCAGGCTGCCCATTTGCAGCGACA23010.10684613970144376No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC22600.10494231887234372No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTAGAGAACCTATGAACATTC22540.10466371094613396No Hit
GGGTAGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22460.10429223371118759No Hit
ATATACCATCGTCCTTTTTGGCGGAGGGACCAGGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA22360.10382788716750466No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC22330.10368858320439978No Hit
GTATCAACATCATCCAGGCTCAGCCCCCAAACTCATTATTTATGATGTCG22220.10317780200634855No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22140.10280632477140218No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22030.10229554357335097No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21880.10159902375782656No Hit
ACATCATCCAGGCTCAGCCCCCAAACTCATTATTTATGATGTCGTTAATCGGCCCTCAGGGATTTCTGATCGCTT21710.10080963463356557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4050.042.1513251
GTATAAC501.9872948E-434.1195833
CGGCAGT22000.034.0478770
CTAGGGA1802.424349E-729.9921270
ATCAACG40100.028.0784593
TCAACGC40550.027.8516484
CAACGCA41700.027.1647515
AACGCAG42950.026.4535966
GAGTTTA7800.025.3880671
CGCAGAG44700.025.341618
GACAGGT3650.025.256151
AGAGTAC45750.024.68255611
TTAACGG1951.8189894E-1224.67950235
CAGAGTA45850.024.63101210
GCAGAGT46650.024.2823169
TAACGGC2001.8189894E-1224.07434536
ACTGGTG43250.022.93617270
TTTATTC9600.022.7469164
TTAAGAC750.002126320322.7463863
CCTATAT18900.022.7463863
Produced by FastQC (version 0.11.5)