FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686138
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19230.28026432000559653No Hit
GTTCAGTACCTGGCCTTCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAAGGAACTGTGGCTGCACCAT17800.25942303151844087No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17510.25519647651055616No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14750.21497133229758444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13550.19748213916150978No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTT13380.19500450346723255No Hit
GTACTGAACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC13160.19179815139228554No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC11210.1633782125461642No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTTCAGTACCTGG11160.1626494961654944No Hit
CTTTTGGCCAGGGGACCAAGCTCGAGATCAAAGGAACTGTGGCTGCACCA10880.15856868443374367No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10640.15507084580652872No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10110.14734645217142908No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9820.14311989716354437No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGACACCAGCGGCCCTGGT9570.13947631526019547No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9510.13860185560339175No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9400.13699867956591824No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9240.13466678714777494No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9190.13393807076710515No Hit
CTCCTGCAGGGCCAGTCGGAGTGTTAGTGGCGGCTTAGCCTGGTACCAAAAGAAGCCTGGCCAGCCTCCCAGGCT9060.13204340817736374No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9060.13204340817736374No Hit
GTACCTGGCCTTCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAA9020.13146043507282792No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8330.12140414901958498No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTGTTAGTGG8180.11921799987757566No Hit
GGCCAAAAGTGTACGAAGGCCAGGTACTGAACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG8090.11790631039237005No Hit
GTTTATTACTGTCAGCAGTTCAGTACCTGGCCTTCGTACACTTTTGGCCA8060.11746908056396818No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7900.11513718814582488No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7880.11484570159355698No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7860.11455421504128907No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTGTTAGTGGCGGCTTAGCCTGGTA7610.1109106331379402No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCGAGCTTGGTCC7220.10522664536871591No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7200.10493515881644801No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCACTGAACCTGGCTGGGACACCA6970.10158306346536702No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG6920.10085434708469725No Hit
GAGTGTTAGTGGCGGCTTAGCCTGGTACCAAAAGAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTACATC6900.10056286053242934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA150.002037939671.56283654
TTTGGCG306.057721E-446.3817243
TGCGTGA502.9346938E-643.05117855
TCGTATG1450.042.85353540
CGTATGC1300.042.5987841
GGTATCA9300.041.248491
ATGCCGT1450.040.8670944
ATCTCGT1700.040.29337737
CGAGCCC3150.039.51684615
TCTCCGA3150.039.5082111
GCGTGAA555.5361234E-639.2533856
CATCTCG1750.039.04651636
GCCCACG3300.038.74292418
ATCTCCG3300.038.73163210
CCCACGA3350.038.16189219
GTATGCC1650.037.86591342
GTATCAA22150.037.8278541
CGAGACT3400.037.6198923
ACGAGAC3450.037.0692622
TCCGAGC3400.036.6058613
Produced by FastQC (version 0.11.5)