FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686138
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34950.5093727500881747No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20580.2999396622836805No Hit
GTTCAGTACCTGGCCTTCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAAGGAACTGTGGCTGCACCAT18000.26233789704112004No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14070.2050607895204755No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13840.2017086941693945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13720.19995977485578703No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTT13230.1928183543252232No Hit
GTACTGAACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC13030.1899034888025441No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11550.16833348393471867No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10830.15783996805307385No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTTCAGTACCTGG10810.15754848150080597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC10440.1521559802838496No Hit
CTTTTGGCCAGGGGACCAAGCTCGAGATCAAAGGAACTGTGGCTGCACCA10100.1472007088952951No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9410.13714442284205217No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9290.13539550352844473No Hit
CTCCTGCAGGGCCAGTCGGAGTGTTAGTGGCGGCTTAGCCTGGTACCAAAAGAAGCCTGGCCAGCCTCCCAGGCT9230.134521043871641No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGACACCAGCGGCCCTGGT9110.13277212455803353No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8850.12898279937855067No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8820.1285455695501488No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8710.1269423935126753No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8360.12184137884798685No Hit
GTACCTGGCCTTCGTACACTTTTGGCCAGGGGACCAAGCTCGAGATCAAA8200.11950948642984356No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8110.11819779694463796No Hit
GTTTATTACTGTCAGCAGTTCAGTACCTGGCCTTCGTACACTTTTGGCCA7820.11397124193675325No Hit
GGCCAAAAGTGTACGAAGGCCAGGTACTGAACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG7720.1125138091754137No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTGTTAGTGG7590.11061914658567226No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7520.10959894365273459No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7300.10639259157778758No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7170.10449792898804613No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCGAGCTTGGTCC7000.1020202932937689No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC6990.10187455001763493No Hit
GAGTGTTAGTGGCGGCTTAGCCTGGTACCAAAAGAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTACATC6980.10172880674150099No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6950.10129157691309912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGCG150.00260454267.2703220
CTAACGC353.942896E-757.6014373
ACGAGAT200.006201201654.0365157
CTCGCTA252.8977837E-453.85948623
TCGCTAT409.772302E-750.50432624
CCTAACG409.886098E-750.4196322
ACGCCAT607.075869E-944.8043866
GGTCGTA404.4855835E-543.92517547
GGTATCA9900.040.072851
GTCGTAA458.899221E-539.107248
GTATCAA24950.038.134611
ATAGCGG502.1719543E-433.6032877
GATACAC502.1728844E-433.6008383
TATCAAC29400.032.3554532
ATCAACG29950.031.8618973
AGGTCGT552.9740925E-431.85089746
AACGCAG30100.031.7054256
CAACGCA30500.031.177175
TCAACGC30650.031.1319484
TCGGGAT553.7700217E-430.58857522
Produced by FastQC (version 0.11.5)