FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421388
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12050.28595973307260764No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11800.28002695852753284No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10070.2389721586756149No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC9980.23683635983938792No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9760.23161551823972204No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9630.22853047547628313No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9400.2230723228948143No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9230.21903803620416337No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAAAGCCAGTGCCCCAGGTCTGACAGTAATACTCAG8950.21239332871367955No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8850.21002021889564962No Hit
GAGTATTACTGTCAGACCTGGGGCACTGGCTTTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG8560.20313820042336278No Hit
GTATTACTGTCAGACCTGGGGCACTGGCTTTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC8430.20005315765992387No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8420.19981584667812086No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8300.19696811489648497No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8240.19554424900566697No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7900.18747567562436518No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7560.1794071022430634No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7250.1720504618071706No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC7140.16944004100733764No Hit
GTAATACTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC6840.16232071155324784No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6730.1597102907534149No Hit
CAGTAATACTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC6710.15923566878980894No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG6590.156387937008173No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6400.1518790283539161No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6380.1514044063903101No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG6260.1485566746086742No Hit
GCTTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCT6260.1485566746086742No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCAGTTGATGCCATGCGATGGCGTAGCTGCTGTGC6130.1454716318452353No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6120.14523432086343227No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAA6040.14333583300900835No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6030.14309852202720533No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGTT6020.14286121104540234No Hit
CAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG6020.14286121104540234No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6010.14262390006359935No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC5890.1397761682819634No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG5870.13930154631835742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.13479263766410055No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5590.1326568388278736No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5540.13147028391885862No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5470.12980910704623766No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5120.12150322268313289No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5100.1210286007195269No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5090.12079128973772389No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5070.12031666777411792No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5060.12007935679231492No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5020.11913011286510293No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC4860.11533313715625505No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT4790.11367196028363408No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC4740.11248540537461911No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT4680.11106153948380115No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC4620.10963767359298318No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT4560.10821380770216524No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA4560.10821380770216524No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT4450.1056033869023323No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC4430.1051287649387263No Hit
GTCTGAGGATGAGGCTGAGTATTACTGTCAGACCTGGGGCACTGGCTTTGTGGTCTTCGGCGGAGGGACCAAG4230.10038254530266642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGG200.00785993750.8895126
GTATCCG200.00788211350.8532625
ACGTACT200.00788211350.8532625
TCGTCAG200.00789321750.83515523
TCGTATG451.8185838E-646.14519540
TAGCTCG306.496598E-445.7240336
TAGACGG350.001471106238.7269484
CTAACGC350.001471106238.7269483
GACGGGT350.001471106238.7269486
GGTATCA7100.038.6587681
GTATCAA17800.038.0742451
CGTATGC557.1471895E-637.81474341
ATGCCGT601.2636014E-534.79868344
ACGAGAC601.5146643E-533.89010222
ATAGACG502.0662871E-433.886083
CCCACGA601.51589575E-533.88607819
GTGGTAT2200.032.3458061
TATCAAC21200.031.9679992
TAGTTCG901.4907528E-731.6930157
TCTCCGA751.9165473E-631.6270111
Produced by FastQC (version 0.11.5)