FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121275
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC61350.5471449911930615No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59340.5292189694767118No Hit
GTATAATAGTTATTCTCCGGAGATGTGCAGTTTTGGCCAGGGGACCAAGC59060.5267218122226929No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50100.4468127800940893No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATCTATAAGGCGT47880.4270138904372255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43220.38545405899534013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35970.3207955229537803No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35600.31749570801096966No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34960.3117879200017837No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32770.29225658290785045No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32570.2904728991549798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32520.29002697821676215No Hit
GATCTATAAGGCGTCTATTTTAGAAAATGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA32040.2857461372098727No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30900.2755791398185102No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA30370.27085237787340305No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA30200.269336246683463No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG29890.2665715368665136No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27950.24926980446366861No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27950.24926980446366861No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27840.24828877839958977No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27740.24739693652315445No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26700.23812178100822723No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26350.23500033444070365No Hit
GTGCAGCCACAGTTCGTTTGATCTGCAGCTTGGTCCCCTGGCCAAAACTG25610.22840070455508238No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25130.2241198635481929No Hit
GTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCA24810.22126596954359992No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24730.22055249604245167No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24660.21992820672894695No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT24430.21787697041314577No Hit
GTATAATAGTTATTCTCCGGAGATGTGCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTG23850.21270428752982098No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT22670.20218055338788432No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22540.2010211589485184No Hit
CAGTATAATAGTTATTCTCCGGAGATGTGCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGG22470.20039686963501369No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22010.19629439700341128No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21790.19433234487525364No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA21280.18978395130543355No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCAGCTTGGTCC20890.18630576798733586No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20760.18514637354796995No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20530.18309513723216872No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC20450.1823816637310205No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20340.18140063766694164No Hit
GGCCAAAACTGCACATCTCCGGAGAATAACTATTATACTGTTGGCAGTAA20200.1801520590399322No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19780.17640632315890392No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19570.17453345521838978No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC19100.17034179839914382No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18500.164990747140532No Hit
TTATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC18460.16463401038995787No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG18380.16392053688880961No Hit
CCTTATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA17680.15767764375376248No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCTCCGGAGATGTGCAGTTT17320.15446701299859536No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17180.15321843437158592No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17020.15179148736928944No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16290.14528104167131167No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16130.1438540946690152No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16110.14367572629372813No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16090.14349735791844107No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16030.14296225279257987No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15850.1413569374149963No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15790.14082183228913514No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15670.13975162203741276No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15600.13912733272390806No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15510.13832467503511628No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15510.13832467503511628No Hit
TCTCCGGAGAATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCA15410.13743283315868096No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC15370.13707609640810683No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATCTATAAGGCGTCTATTTTAGAAAATGGGGTCCCAT15190.1354707810305233No Hit
CTCCGGAGATGTGCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGA15170.13529241265523623No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15080.13448975496644444No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC14880.13270607121357383No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14850.13243851865064324No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14200.12664154645381376No Hit
GTGCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTG14200.12664154645381376No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14160.12628480970323963No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14130.126017257140309No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14120.1259280729526655No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14080.12557133620209138No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT14070.12548215201444785No Hit
TCTATAAGGCGTCTATTTTAGAAAATGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT13930.1242335733874384No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13830.12334173151100311No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13820.12325254732335957No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13810.12316336313571603No Hit
TAGTTATTCTCCGGAGATGTGCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCAT13280.1184366011906089No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCGTGATGGGACCCCA12970.11567189137365946No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12870.11478004949722415No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12710.11335310249492765No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG12660.11290718155670999No Hit
GGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12620.11255044480613587No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12550.11192615549263116No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12510.11156941874205703No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12500.1114802345544135No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12340.110053287552117No Hit
GATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC12320.10987491917682995No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12290.10960736661389935No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12210.1088938931127511No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11810.10532652560700988No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11630.10372121022942632No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG11620.1036320260417828No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11560.10309692091592161No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11280.10059976366190275No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11250.10033221109897215No Hit
GTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATCTATAAGG11240.10024302691132862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG252.9558362E-453.646572
TCGTATG3000.052.7887640
ATGCCGT3150.051.92202844
GCCGTCT3100.051.89640846
CACGAGA3200.051.36205721
CGTATGC3100.051.21726241
ACGAGAC3300.049.81452622
CCCACGA3300.049.781219
TCTCCGA3450.047.61467711
GTGGTAT2350.047.0834241
ATCTCGT3000.046.68522337
CGAGACC3600.046.61186223
CCGTCTT3500.045.07519547
TATACAC3800.044.9995883
GTATGCC3700.044.90326342
GCCCACG3800.042.3468918
TCCGAGC3900.042.11879713
CTAACGC405.9193633E-541.9113773
TGCTTGA3950.041.83839455
CTTATAC4100.040.8891531
Produced by FastQC (version 0.11.5)