FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160066
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35370.3048964455470637No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34720.29929331606994775No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCGTCAGGAAGA32270.27817382804081836No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29130.2511064025667505No Hit
GGCTAACAGTTTCCCCCTCGCTTTCGGCGGTGGGACCAGGGTGGAGATCAAGGGAACTGTGGCTGCACCAT25300.21809103964774418No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24180.20843641654871362No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23330.20110924723248505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21940.18912717035065246No Hit
GATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCTAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA21100.18188620302637953No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20870.17990355721140003No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20740.17878293131597683No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20610.17766230542055364No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20200.17412802375037284No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20150.17369701379059466No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18760.1617149369087621No Hit
GGCTATTAGTAGTTGGTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCGTC18650.16076671499725015No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18410.15869786719031503No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18120.15619800942360176No Hit
CTGTTAGCCTGCTGACAATAGTAAGTTGCAAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC17790.15335334368906597No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17460.15050867795453016No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16980.14637098234065993No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16360.14102645883941087No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTACTGGAGCTGGATCCGGCAGCC16080.1386128030646532No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15870.136802561233585No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15850.13663015724967373No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15300.13188904769211407No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15290.13180284570015843No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14920.12861337199780012No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14710.1268031301667319No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14590.12576870626326433No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14550.12542389829544182No Hit
CTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCTAGGTT14490.12490668634370804No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14430.12438947439197426No Hit
CATCTAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATTTTG14330.12352745447241796No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT13710.11818293097116887No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13630.11749331503552385No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13540.11671749710792317No Hit
GTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAGTAGTTGGTTAGGCTGGTATCAGCAGAAA13400.11551066922054434No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATTTTGCAACTTACTATTGTCAGCAGGCTAACAGTTTC13150.1133556194216536No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT12950.111631579582541No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATCTCCACCCTGGTCCCACCGCCGAAAGCGAGGGGGAAA12710.10956273177560587No Hit
GTCTATTACTGTGCGAGGCGTGGGAGCTTCTCCCGGGCCTTTATGGACGT12640.10895931783191645No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12370.10663186404911444No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC12120.10447681425022369No Hit
GTGCAGCCACAGTTCCCTTGATCTCCACCCTGGTCCCACCGCCGAAAGCG12110.10439061225826805No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11860.10223556245937732No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11770.10145974453177665No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11730.10111493656395412No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11710.10094253258004285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTCG200.003338233363.18878669
CCCGTTA200.00703230852.34776349
GTGGTAT3450.044.6032371
GTATCAA36750.036.8477361
TATCAAC41150.032.9020842
ATCAACG42600.031.8610613
TCAACGC42700.031.7063774
CGCTAAT1305.456968E-1231.68833232
TAGTGAT9800.031.52239670
CAACGCA43150.031.4549525
TCGTATG1450.030.96539140
AACGCAG44100.030.93246
CGTATGC1501.8189894E-1229.98152241
ACGCAGA46050.029.6200077
CGGGTAC1401.6370905E-1129.30816817
CGCAGAG46500.029.264898
TGTGTCG16900.028.35150370
AGAGTAC48650.028.04548811
CAGAGTA48750.027.91660910
GCAGAGT49450.027.5202439
Produced by FastQC (version 0.11.5)