FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160066
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34970.3014483658688385No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27780.23946913365274045No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27360.23584864999060395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24760.21343613208214016No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24120.20791920459697985No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCGTCAGGAAGA24050.20731579065329042No Hit
GGCTAACAGTTTCCCCCTCGCTTTCGGCGGTGGGACCAGGGTGGAGATCAAGGGAACTGTGGCTGCACCAT23360.20136785320835193No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22030.18990298827825314No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21200.18274822294593582No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20760.1789553352998881No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20200.17412802375037284No Hit
CTGTTAGCCTGCTGACAATAGTAAGTTGCAAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC19820.17085234805605887No Hit
GATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCTAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA19430.1674904703697893No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC18690.16111152296507267No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18440.15895647316618192No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18340.1580944532466256No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18270.15749103930293623No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTACTGGAGCTGGATCCGGCAGCC17760.15309473771319906No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17210.14835362815563943No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16330.14076785286354399No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16320.14068165087158835No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16050.13835419708878632No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16010.1380093891209638No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15770.1359405413140287No Hit
GGCTATTAGTAGTTGGTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCGTC15680.13516472338642802No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15590.13438890545882734No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15570.1342165014749161No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14840.1279237560621551No Hit
CTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCTAGGTT14610.12594111024717558No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14340.12361365646437357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14280.12309644451263979No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT13950.120251778778104No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13900.11982076881832586No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13440.11585547718836688No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATTTTGCAACTTACTATTGTCAGCAGGCTAACAGTTTC13400.11551066922054434No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13280.1144762453170768No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG13130.11318321543774233No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATCTCCACCCTGGTCCCACCGCCGAAAGCGAGGGGGAAA12990.1119763875503635No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12840.11068335767102906No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12660.10913172181582771No Hit
CATCTAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATTTTG12240.10551123815369125No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12060.10395960229848993No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11990.10335618835480051No Hit
GGGTGGAGATCAAGGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG11880.10240796644328856No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11830.10197695648351043No Hit
GTGCAGCCACAGTTCCCTTGATCTCCACCCTGGTCCCACCGCCGAAAGCG11660.1005115226202647No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11650.10042532062830907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC200.00770544751.15147
TAGTGAT9050.042.84874370
GTGGTAT3600.039.80331
GTACGGT350.001423822738.9909861
GTATCAA35350.036.9642261
TCGGACG400.002394784235.07308652
TATCAAC41400.031.6366732
TCAACGC42650.030.8614184
ATCAACG42800.030.751933
GTATAGA1001.616354E-830.70541
GCGAACT902.1443157E-730.4155228
CTAGTGA8550.030.24931169
CAACGCA43500.030.181295
TTAACGG1151.7589628E-929.91086235
AACGCAG44300.029.7902076
CGGGTAG704.1495827E-529.2495517
GCACGAG900.006362476428.72452770
CGAACTA854.698568E-628.19616129
ACGCAGA46850.028.1687567
CGCAGAG46850.027.9503948
Produced by FastQC (version 0.11.5)