FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470713
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATTACTGTGCAGCATGGGATGACAGCCTGAGTGCTCGGGTGTTCGG130390.886576782825745No Hit
GTAATAAATAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG103190.7016324735009483No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACTCAGGCTGTCATCCCATG68480.4656244964177239No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC67780.46086490022186516No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC59660.40565358434990373No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG57400.390286888060417No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50500.3433708684155236No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48550.33011199329848856No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC45440.3089657873426019No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT44910.3053620930800231No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43540.2960468833824138No Hit
GATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG42660.2900633910219057No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA41520.2823120486457929No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC41030.27898033130869176No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG39940.271568960089426No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACTCAGG39760.27034506392477664No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG39670.26973311584245196No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT39270.2670133465876755No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG38510.2618457850036003No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT37880.25756214842732744No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG37640.25593028687446157No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36070.2452551925494641No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35650.24239943483194887No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC35400.24069957904771358No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35050.2383197809497842No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33970.23097640396188787No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32770.22281709619755857No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31150.21180203071571407No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT30880.20996618646874No Hit
CCCCTGAGGGCCGCTGATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA30840.20969420954326237No Hit
GCATGGGATGACAGCCTGAGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG30280.20588653258657535No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC30180.20520659027288124No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30170.20513859604151183No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG28630.19466748441062262No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28410.19317161132049557No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA28360.1928316401636485No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28030.19058783052845796No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27160.18467233239931924No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACTCAGGC26730.1817485804504346No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT25540.1736572669174747No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT25440.17297732460378062No Hit
CCCGAGCACTCAGGCTGTCATCCCATGCTGCACAGTAATAAATAGCCTCATCCTCGGACCGGAGCCCACTGATGG25330.1722293880587171No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24940.16957761303531008No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24930.16950961880394066No Hit
GTAATAGACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA24550.16692583801190308No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC24390.1658379303099925No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC23330.158630541784835No Hit
GTGCAGCATGGGATGACAGCCTGAGTGCTCGGGTGTTCGGCGGAGGGACC22880.1555708013732115No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG22690.1542789109771927No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT22520.1531230090439127No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22520.1531230090439127No Hit
CTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC22090.15019925709502804No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG21360.1452356782050611No Hit
ATCCCATGCTGCACAGTAATAAATAGCCTCATCCTCGGACCGGAGCCCAC21360.1452356782050611No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21320.14496370127958344No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTATTTATTACTGTGCAG21140.14373980511493406No Hit
ATGCTGCACAGTAATAAATAGCCTCATCCTCGGACCGGAGCCCACTGATG21090.143399833958087No Hit
GTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC20960.14251590895028465No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG20690.14068006470331057No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20620.1402041050837247No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20610.14013611085235528No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC20560.13979613969550825No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20540.1396601512327694No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20370.13850424929948943No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20330.13823227237401178No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT20160.13707637044073182No Hit
GGATGACAGCCTGAGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19810.13469657234280244No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC19800.13462857811143303No Hit
GTCTATTACTGTGCGAGAGATTTAGCCTCCAGAGTGGCTGATATCTGGGG19460.13231677424487306No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19320.1313648550057013No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18790.12776116074312255No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18590.12640127611573435No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG18370.12490540302560732No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18270.1242254607119132No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17930.12191365684535323No Hit
GTCCGAGGATGAGGCTATTTATTACTGTGCAGCATGGGATGACAGCCTGAGTGCTCGGGTGTTCGGCGGAGGGAC17740.12062176644933444No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17580.11953385874742388No Hit
GCTTGGTCCCTCCGCCGAACACCCGAGCACTCAGGCTGTCATCCCATGCTGCACAGTAATAAATAGCCTCATCCT17390.11824196835140506No Hit
GTATACATAATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA17320.11776600873181918No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17250.11729004911223333No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17230.11715406064949449No Hit
TTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA17160.11667810102990862No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG17070.11606615294758393No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16870.11470626832019572No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16770.1140263260065016No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16740.11382234331239337No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC16720.11368635484965456No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACTCAGGCTGTCATCCCATGCTGCACAGTAAT16620.11300641253596044No Hit
CCCAGGAACGGCCCCCAAACTCCTCATGTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG16530.11239446445363574No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG16500.11219048175952753No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC16470.1119864990654193No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG16150.10981068366159816No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACC16110.10953870673612051No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACATAATTACTT16020.10892675865379581No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15910.1081788221087323No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC15790.10736289133229937No Hit
CTCAGGCTGTCATCCCATGCTGCACAGTAATAAATAGCCTCATCCTCGGA15780.10729489710092996No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15760.10715890863819114No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15480.10525507015984763No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15340.10430315092067588No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTATT15310.10409916822656765No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG15000.10199134705411593No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14990.10192335282274653No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAG14980.10185535859137711No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG14900.10131140474042181No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT14810.10069945665809714No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14730.10015550280714183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)