FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168685
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT12090.7167205145685746No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC5120.3035243204789993No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5050.2993745739099505No Hit
GTGTGTACCTTGCATGCAGTAGTAAACCCCACCAACATCCTCAGCCTCCA4850.2875181551412396No Hit
GGTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT4220.2501704360198002No Hit
GTAGTAAACCCCACCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTG4150.2460206894507514No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC3700.21934374722115185No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAAACCCTCCTA3690.2187509262827163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.20452322376026322No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT3450.20452322376026322No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG3270.19385244686842337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.19325962592998785No Hit
GTTTACTACTGCATGCAAGGTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA3120.18496013279189022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.18318166997658358No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3090.18318166997658358No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG2950.17488217683848595No Hit
CCTACACACTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG2910.17251089308474374No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT2830.1677683255772594No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2740.1624329371313395No Hit
GGCCAGGCCAATCTCCAAGGCGCCTCATTTATCAGGTTTCTAAGTGGGAC2560.15176216023949965No Hit
GTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA2520.14939087648575747No Hit
CTCCTACACACTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC2500.1482052346088864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.14642677179357974No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2450.14524112991670865No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT2450.14524112991670865No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.13457035302486883No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA2220.1316062483326911No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG2170.12864214364051338No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC2160.12804932270207783No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2150.1274565017636423No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG2140.12686368082520674No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2110.1250852180099001No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2080.12330675519459347No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG2070.12271393425615792No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT2060.12212111331772238No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.12034265050241576No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC1980.11737854581023802No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC1980.11737854581023802No Hit
GGCCAAAAGTGAACGGCCAGTGTGTACCTTGCATGCAGTAGTAAACCCCACCAACATCCTCAGCCTCCACCCTG1980.11737854581023802No Hit
GTCCAAGGGTGACGGCCAGGGAGAGTGGAGACTGAGTCATCACAACATCC1960.11619290393336693No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.11619290393336693No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC1940.11500726205649583No Hit
AGGTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT1940.11500726205649583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.11026469454901147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.10967187361057593No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.10848623173370484No Hit
CTCCAAGGCGCCTCATTTATCAGGTTTCTAAGTGGGACTCTGGGGTCCCA1800.1067077689183982No Hit
GTCTCCACTCTCCCTGGCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAAACCCTCCTACA1800.1067077689183982No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA1790.10611494797996267No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC1780.1055221270415271No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT1750.10374366422622047No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGGCGT1750.10374366422622047No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT1750.10374366422622047No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1740.10315084328778493No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG1730.10255802234934938No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA1700.10077955953404275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)