FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168685
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91355.415419272608708No Hit
GTGTGTACCTTGCATGCAGTAGTAAACCCCACCAACATCCTCAGCCTCCA4960.29403918546403063No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT4520.26795506417286663No Hit
GGTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT4340.25728428728102676No Hit
GTAGTAAACCCCACCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTG4020.23831401725108928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.21578682159053858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.21578682159053858No Hit
GTTTACTACTGCATGCAAGGTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA3460.20511604469869876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.2003734771912144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC3270.19385244686842337No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG3200.18970270029937458No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3180.1885170584225035No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAAACCCTCCTA3100.18377449091501913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.17369653496161483No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2750.16302575806977504No Hit
CTCCTACACACTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC2640.156504727746984No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT2620.15531908587011295No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2530.149983697424193No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG2520.14939087648575747No Hit
GGCCAGGCCAATCTCCAAGGCGCCTCATTTATCAGGTTTCTAAGTGGGAC2430.14405548803983756No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2380.14109138334765986No Hit
GTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA2290.13575599490173992No Hit
CCTACACACTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG2280.13516317396330438No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.13042060645582002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT2170.12864214364051338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.1274565017636423No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT2150.1274565017636423No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.12686368082520674No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT2050.12152829237928683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.11797136674867356No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC1980.11737854581023802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1980.11737854581023802No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1980.11737854581023802No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG1980.11737854581023802No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC1980.11737854581023802No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.11619290393336693No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC1950.11560008299493138No Hit
GTCTCCACTCTCCCTGGCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAAACCCTCCTACA1950.11560008299493138No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC1940.11500726205649583No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1940.11500726205649583No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC1930.11441444111806029No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.11322879924118921No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.11145033642588256No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.11026469454901147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG1860.11026469454901147No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA1850.10967187361057593No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT1820.10789341079526929No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG1820.10789341079526929No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG1790.10611494797996267No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA1780.1055221270415271No Hit
AGGTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT1720.10196520141091384No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT1710.10137238047247829No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA1700.10077955953404275No Hit
GTCCAAGGGTGACGGCCAGGGAGAGTGGAGACTGAGTCATCACAACATCC1690.1001867385956072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)