FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009128
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACTGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTTAGGGAAT118560.5901067527803107No Hit
GTCTATTACTGTGCGAGAGGATCACGGGAGAGGTACTTCGATCTCTGGGG79670.3965401905702375No Hit
GTCCCTATCCTCGTGATCCAAAAGGCGTCTACTTTAGAAAGTGGGGTCCC65040.323722530371385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53370.26563762985733114No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49130.24453394706559264No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG43960.2188013904539681No Hit
GTATAGTCGTTTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAGCGAACTGTGGCTGCACCAT43730.21765661520818982No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA41930.20869750458905556No Hit
CTATTGGAGTTGGATCCGCCAGGCCCCAGGGAAGGGGCTGGAGTGGATTGGTGAAGTCAATCATGTTGGAAGTAC41630.2072043194858665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39050.1943629275984407No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCTGCTCCCAGATGGGTC38130.18978382661532764No Hit
CACTATCACCAGCCTGCAGCCTGCTGATTATGCAACTTATTACTGCCAACAGTATAGTCGTTTTCCGTGGACGTT37000.18415949605998225No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT36650.18241744677292834No Hit
GAGTATTAATGGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGTCCCTATCCTCGTGATCCAAAAGGCGT35800.17818675564722605No Hit
GTATTAATGGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGTCCCTATCCTCGTGATCCAAAAGGCGTCTA35600.1771912989117667No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34020.16932719070163774No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34000.1692276450280918No Hit
CAGTAATAGACTGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTTAGGGAATACTGGTTCTCGGACGTGTCTA32940.16395172433015717No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC32500.16176171951214657No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG31710.15782966540708207No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG31530.15693375434516865No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30850.15354920144460682No Hit
CTATTACTGTGCGAGAGGATCACGGGAGAGGTACTTCGATCTCTGGGGCC30740.15300170024010418No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30630.15245419903560153No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30600.15230488052528263No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC30590.15225510768850964No Hit
CACCTGCACTGTCGACGGTGGGTCTTTCGGTGATAACTATTGGAGTTGGATCCGCCAGGCCCCAGGGAAGGGG30150.15006510287049904No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29630.1474769153583047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29530.14697918699057502No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT29470.1466805499699372No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT28760.14314667855905647No Hit
CCCTAAAGCTGACCTCTGTGACCGCCGCGGACACGGCAGTCTATTACTGT28470.14170326629264038No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATGGGTGGTTGGCCTGGTATCAGCAGAAA28040.13956303431140277No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC28010.13941371580108386No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27820.13846803190239745No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27410.13642734559470576No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27350.13612870857406797No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA27310.13592961722697608No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG27270.1357305258798842No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27130.13503370616506266No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC26950.13413779510314922No Hit
ACTTATTACTGCCAACAGTATAGTCGTTTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAGCGAA26310.13095233354967925No Hit
CGACTATACTGTTGGCAGTAATAAGTTGCATAATCAGCAGGCTGCAGGCTGGTGATAGTGAGAGTGAATTCTGTC26310.13095233354967925No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC25940.12911073858907945No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25870.12876232873166865No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC25770.128264600363939No Hit
GTCGACGGTGGGTCTTTCGGTGATAACTATTGGAGTTGGATCCGCCAGGC25590.12736868930202555No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG25590.12736868930202555No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG25490.12687096093429587No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25430.12657232391365805No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG25260.1257261856885176No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24880.12383481789114481No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24770.12328731668664217No Hit
GATAGGGACTGTCCCTGGTTTCTGCTGATACCAGGCCAACCACCCATTAA24550.12219231427763685No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT24190.12040049215381No Hit
GGACCAAGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC24160.12025117364349111No Hit
GCTTTAGGGAATACTGGTTCTCGGACGTGTCTACTGATATGGTGACTCGA24140.12015162796994516No Hit
GAATTCACTCTCACTATCACCAGCCTGCAGCCTGCTGATTATGCAACTTATTACTGCCAACAGTATAGTCGTTTT23580.11736434911065895No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT23520.11706571209002113No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAAAACGACTATACTGTTGGCAGTAATAAGTTGCATAATCAGCAGG23260.11577161833392396No Hit
CTTCAAGGTTTAGCGGCAGTGGATATGGGACAGAATTCACTCTCACTATCACCAGCCTGCAGCCTGCTGATTATG23220.11557252698683211No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22850.11373093202623227No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC22690.11293456663786479No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACAGTGACCTGGGTGCCACGG22670.11283502096431884No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC22540.11218797408627026No Hit
GTCCTGTCCCAGGTGCAGCTACAGGAGGGGGCCACAGGAGCGGTGAAGCC22390.11144138153467574No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC22100.10999796926825967No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT21500.10701159906188157No Hit
GTCCCTCACCTGCACTGTCGACGGTGGGTCTTTCGGTGATAACTATTGGA21490.10696182622510861No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATGTCCACCTTGGTCC21420.10661341636769783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21160.10531932261160065No Hit
ACTTTAGAAAGTGGGGTCCCTTCAAGGTTTAGCGGCAGTGGATATGGGACAGAATTCACTCTCACTATCACCAGC20600.10253204375231444No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20500.10203431538458474No Hit
CCATATCAGTAGACACGTCCGAGAACCAGTATTCCCTAAAGCTGACCTCT20470.10188499687426585No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG20460.10183522403749289No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20160.10034203893430384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT353.293626E-759.1292739
TACTGAT31450.051.00612370
GTATCGC451.9073232E-645.83997335
ATCGCGT503.8943235E-641.3220937
TATCGCG503.914476E-641.29107336
TCGTATG1600.038.83572840
GTCTCGT28300.037.26827270
CGTATGC1950.035.4449441
GTATCAA36350.035.1353261
GTGGTAT3900.035.118421
ACCGATC2950.034.8433627
GCGTATG601.3375498E-534.52064540
TCTACTG29400.033.86406768
ATCTCGT2150.033.6342637
GACCGAT3100.033.14665626
TGTCTAC28750.033.05668366
ATCTCCG3250.032.64631310
TATACAC3950.032.0645563
CTACTGA34500.031.55853869
GACAGGT5050.031.1893271
Produced by FastQC (version 0.11.5)