FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009128
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACTGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTTAGGGAAT105270.523958652709036No Hit
GTCTATTACTGTGCGAGAGGATCACGGGAGAGGTACTTCGATCTCTGGGG78350.38997017611620566No Hit
GTCCCTATCCTCGTGATCCAAAAGGCGTCTACTTTAGAAAGTGGGGTCCC60210.2996822502100414No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53980.2686737729004822No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49490.2463257691894195No Hit
GTATAGTCGTTTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAGCGAACTGTGGCTGCACCAT43010.2140729709605361No Hit
CTATTGGAGTTGGATCCGCCAGGCCCCAGGGAAGGGGCTGGAGTGGATTGGTGAAGTCAATCATGTTGGAAGTAC41820.2081500033845529No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39880.1984940730505971No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39660.19739907064159176No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCTGCTCCCAGATGGGTC36830.1833133578348418No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG35500.17669357054403703No Hit
CACCTGCACTGTCGACGGTGGGTCTTTCGGTGATAACTATTGGAGTTGGATCCGCCAGGCCCCAGGGAAGGGG35190.175150612604075No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA34810.1732592448067022No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT34240.17042219311064305No Hit
GAGTATTAATGGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGTCCCTATCCTCGTGATCCAAAAGGCGT33950.16897878084422693No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC32780.16315535894178967No Hit
GTATTAATGGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGTCCCTATCCTCGTGATCCAAAAGGCGTCTA32690.16270740341083295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31090.15474374952715805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30840.15349942860783386No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30620.15240442619882855No Hit
CACTATCACCAGCCTGCAGCCTGCTGATTATGCAACTTATTACTGCCAACAGTATAGTCGTTTTCCGTGGACGTT30490.15175737932077996No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG29660.1476262338686236No Hit
CAGTAATAGACTGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTTAGGGAATACTGGTTCTCGGACGTGTCTA29600.1473275968479858No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA29150.1450878191932022No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG28800.14334576990614833No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28760.14314667855905647No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28520.14195213047650523No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC28090.1398118984952676No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC27780.1382689405553056No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27640.13757212084048404No Hit
CTATTACTGTGCGAGAGGATCACGGGAGAGGTACTTCGATCTCTGGGGCC27390.13632779992115981No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26340.13110165205999816No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC26270.13075324220258738No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25790.12836414603748492No Hit
ACTTATTACTGCCAACAGTATAGTCGTTTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAGCGAA25610.1274682349755715No Hit
CGACTATACTGTTGGCAGTAATAAGTTGCATAATCAGCAGGCTGCAGGCTGGTGATAGTGAGAGTGAATTCTGTC25530.12707005228138776No Hit
CCCTAAAGCTGACCTCTGTGACCGCCGCGGACACGGCAGTCTATTACTGT25240.12562664001497167No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC25170.1252782301575609No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25080.12483027462660418No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24740.12313799817632326No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATGGGTGGTTGGCCTGGTATCAGCAGAAA24250.1206991291744478No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24180.12035071931703704No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT24120.12005208229639924No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG24050.11970367243898845No Hit
GTCGACGGTGGGTCTTTCGGTGATAACTATTGGAGTTGGATCCGCCAGGC23660.11776253180484271No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23660.11776253180484271No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23370.11631911953842661No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC23360.11626934670165366No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG23200.11547298131328615No Hit
GATAGGGACTGTCCCTGGTTTCTGCTGATACCAGGCCAACCACCCATTAA23090.1149254801087835No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACAGTGACCTGGGTGCCACGG23000.1144775245778268No Hit
GCTTTAGGGAATACTGGTTCTCGGACGTGTCTACTGATATGGTGACTCGA22950.11422866039396196No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG22930.11412911472041601No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT22920.11407934188364306No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22820.11358161351591337No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22780.11338252216882151No Hit
GGACCAAGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC22710.11303411231141072No Hit
GTCCCTCACCTGCACTGTCGACGGTGGGTCTTTCGGTGATAACTATTGGA22690.11293456663786479No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22560.1122875197598162No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAAAACGACTATACTGTTGGCAGTAATAAGTTGCATAATCAGCAGG22260.11079433465662715No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22190.11044592479921639No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC22130.11014728777857857No Hit
GAATTCACTCTCACTATCACCAGCCTGCAGCCTGCTGATTATGCAACTTATTACTGCCAACAGTATAGTCGTTTT22100.10999796926825967No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21410.10656364353092485No Hit
GGGCTGGAGTGGATTGGTGAAGTCAATCATGTTGGAAGTACCTTCTACAA21220.10561795963223847No Hit
GTATCAACGCAGAGTACGGGGTCCTTTCTGAGAGTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTT21200.10551841395869253No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21050.10477182140709801No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20570.10238272524199553No Hit
CTTCAAGGTTTAGCGGCAGTGGATATGGGACAGAATTCACTCTCACTATCACCAGCCTGCAGCCTGCTGATTATG20530.10218363389490365No Hit
GTCCTGTCCCAGGTGCAGCTACAGGAGGGGGCCACAGGAGCGGTGAAGCC20490.1019845425478118No Hit
ACTTTAGAAAGTGGGGTCCCTTCAAGGTTTAGCGGCAGTGGATATGGGACAGAATTCACTCTCACTATCACCAGC20460.10183522403749289No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT20340.10123794999621727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)