FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719093
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGAGACA25070.34863362597049335No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23180.3223505165534917No Hit
GTATGTGGGTGGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC21960.3053846998927816No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACCACCCACATACAATACA21660.3012127777630988No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG20560.28591572995426184No Hit
CATCTACACCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC20420.2839688329604098No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20050.27882346233380106No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20040.2786843982628116No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACCACCCACATACAATACACAGTAATAAT19780.2750687324170865No Hit
CCCACATACAATACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC19670.2735390276362028No Hit
GTGTAGATGAGCGTGAGTGGAGTCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTTACTAGGAGAGACTGAG19600.27256557913927687No Hit
GATTATTACTGTGTATTGTATGTGGGTGGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT19100.2656123755898055No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18710.26018887682121783No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT17850.2482293667161271No Hit
GTATTGTATGTGGGTGGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG17390.24183241945061346No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAACTCTGG16920.23529640811411043No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16190.22514473093188225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16030.22291970579605142No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15380.2138805411817387No Hit
GTGTATTACTGTGCGAAAGGGGGCCTACGCGGGTCTTACTACTTTGACTA15000.20859610648414043No Hit
CCCCAGAAGAGCGAGTGTTTGTGGTGTAGATGAGCGTGAGTGGAGTCTGG14990.20845704241315102No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14820.20609295320633075No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14710.20456324842544707No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14680.20414605621247878No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14430.2006694544377431No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13990.19455063531420833No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13650.18982245690056782No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT13280.184677086273959No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12760.1774457545825088No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12200.1696581666071009No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12060.1677112696132489No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11960.16632062890335464No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11660.16214870677367182No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG11650.1620096427026824No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11620.16159245048971413No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11550.16061900199278814No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11540.1604799379217987No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11520.16020180977981985No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11230.15616895172112646No Hit
CCCCAGGCCAGACTCCACTCACGCTCATCTACACCACAAACACTCGCTCT10810.15032826073957054No Hit
CATACAATACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTG10610.147546979319782No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10590.14726885117780314No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10130.14087190391228951No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA10100.14045471169932122No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT10060.1398984554153635No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG9820.13656091771161727No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9800.13628278956963844No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9740.13544840514370185No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9680.1346140207177653No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAACTCTGGCTCAGTC9650.134196828504797No Hit
CTCCTAGTAACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGACTCCACTCACGCTCATCTACACCACAAACA9630.13391870036281817No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9520.13238899558193445No Hit
GTGTATTGTATGTGGGTGGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG9030.12557485610345254No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTTACTAGGAGAGACTGAG8810.12251544654168516No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8790.1222373183997063No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGCTAC8740.12154199804475915No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGAGA8650.12029042140585432No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC8610.11973416512189661No Hit
CACTTGTGGCTTGAACTCTGGCTCAGTCTCTCCTAGTAACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGA8540.11876071662497063No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8500.11820446034101291No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8430.11723101184408694No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8070.11222470528846756No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8010.111390320862531No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC7970.11083406457857328No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC7970.11083406457857328No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7930.11027780829461559No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT7920.11013874422362616No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACCAC7890.10972155201065788No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC7800.10846997537175301No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC7640.1062449502359222No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7570.10527150173899621No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG7460.10374179695811252No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7240.10068238739634511No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7240.10068238739634511No Hit
CCCAAATGCCACCACCCACATACAATACACAGTAATAATCAGATTCATCA7210.10026519518337683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACC150.00240589168.630335
CCAACCG252.3990676E-455.95954
TCTGGAT8400.052.8721370
GGTACGC200.00751585851.472758
GGTATCA4750.051.288581
GGTCTAC306.2105095E-446.14834242
GTATCAA14350.042.3230821
ATACGTA350.001383535839.2173411
ACGTACC350.00138448239.21188413
TATCAAC15350.039.118812
CAACGCA16100.037.085965
ATCAACG16250.036.7410743
TCAACGC16200.036.6426624
AACGCAG16300.036.6334656
ACGCAGA16950.035.2261927
CGCAGAG17250.034.6135648
AATACGT400.002664971234.31516610
AGAGTAC17600.034.12019711
CAGAGTA17850.033.4500810
GCAGAGT18250.032.716939
Produced by FastQC (version 0.11.5)