FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719093
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGAGACA23360.3248536698313014No Hit
GTATGTGGGTGGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC22020.3062190843187182No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20930.29106110058087065No Hit
CATCTACACCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC20090.27937971861775873No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19640.27312183542323454No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAACTCTGG19590.2724265150682874No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT18240.2536528654847148No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACCACCCACATACAATACA18010.25045439185195795No Hit
GATTATTACTGTGTATTGTATGTGGGTGGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT17730.246560597864254No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG17150.23849488174686723No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17010.23654798475301525No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACCACCCACATACAATACACAGTAATAAT16590.23070729377145932No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16320.2269525638547448No Hit
GTATTGTATGTGGGTGGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16200.22528379500287168No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16090.22375409022198797No Hit
GTGTAGATGAGCGTGAGTGGAGTCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTTACTAGGAGAGACTGAG16050.22319783393803028No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14820.20609295320633075No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14230.19788817301795458No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14150.19677566045003914No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT14020.1949678275271766No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13710.19065684132650437No Hit
GTGTATTACTGTGCGAAAGGGGGCCTACGCGGGTCTTACTACTTTGACTA13570.1887099443326524No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13130.1825911252091176No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12650.17591604980162512No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12610.1753597935176674No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12480.17355196059480485No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG12410.17257851209787886No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12070.16785033368423835No Hit
CCCCAGAAGAGCGAGTGTTTGTGGTGTAGATGAGCGTGAGTGGAGTCTGG11700.16270496305762955No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11640.16187057863169296No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11580.1610361942057564No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10930.15199702959144368No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10840.15074545295253883No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG10820.15046732481055997No Hit
CTCCTAGTAACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGACTCCACTCACGCTCATCTACACCACAAACA10790.15005013259759167No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10680.148520427816708No Hit
CCCCAGGCCAGACTCCACTCACGCTCATCTACACCACAAACACTCGCTCT10630.14782510746176086No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10340.14379224940306748No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10040.13962032727338466No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT9810.13642185364062784No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9740.13544840514370185No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA9690.13475308478875472No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9440.13127648301401904No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC9180.12766081716829394No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9140.12710456088433625No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAACTCTGGCTCAGTC9040.12571392017444197No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9000.12515766389048424No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8590.11945603697991775No Hit
CACTTGTGGCTTGAACTCTGGCTCAGTCTCTCCTAGTAACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGA8490.11806539627002349No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT8440.11737007591507635No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8410.11695288370210807No Hit
CATACAATACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTG8300.11542317892122436No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC8250.11472785856627725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8170.11361534599836183No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8150.11333721785638297No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC8120.11292002564341469No Hit
GTGTATTGTATGTGGGTGGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG8110.11278096157242526No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8050.1119465771464887No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7990.11111219272055214No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTTACTAGGAGAGACTGAG7950.11055593643659445No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7920.11013874422362616No Hit
CCCACATACAATACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC7850.10916529572670015No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGAGA7720.10735746280383762No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7470.10388086102910193No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG7400.10290741253217595No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7340.10207302810623939No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGCTAC7280.10123864368030283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAT6200.054.94608770
TTAGCCC200.007588105751.3483474
GGTATCA4750.044.7380751
GTATCAA13050.041.497871
GTGGTAT1500.038.8451581
TATCAAC14600.036.8344232
ATCAACG15300.034.9155923
GCTAGGA400.00245734634.8873752
GTGAGCG2850.034.8634570
ATCGGAA501.9304459E-434.2846926
TCAACGC15450.034.1214454
AACGCAG15750.033.688866
CAACGCA15650.033.685395
TGGCGCG751.7213715E-632.0705331
GGCGCGT652.3794099E-531.72918132
CGATTCA4700.031.71090570
TGGTATC1950.031.6187762
ACGGGTA652.43911E-531.61437416
GGATGGG6750.031.54312570
CTCTGGA6500.031.27895469
Produced by FastQC (version 0.11.5)