FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990764
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCGGCTCTCAGACTGCTCATTTGCAGATACA31420.31712900347610534No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25390.2562668809121042No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23240.23456645578563612No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT22650.22861145540209374No Hit
GTTTATTACTGTGCGAAGGATGACGTAGCAGTGACTGGTATGGGTCACCT22400.22608815015483No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21940.22144526849986476No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20980.21175577635037202No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20640.20832408121409335No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20150.20337840292945644No Hit
GTGTGAGGTGCAGCTGTTGGAGTCGGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC18790.18965162238434177No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18780.18955069017445123No Hit
GGCCAGGCCAGTCCCCTGTGTTGGTCATCTATCAAGATACTAAGCGGCCC18720.18894509691510794No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA17940.1810723845436451No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17650.17814535045681917No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17620.17784255382714753No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17590.1775397571974759No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17360.17521831636999324No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16680.1683549260974359No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG16590.16744653620842098No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16380.16532695980071946No Hit
GGGCTAGAGTGGGTCTCAGCTGTCAGTGGCAGTGGTAGTAGGACATACTA16230.1638129766523612No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15790.15937195941717705No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15660.1580598406885999No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15640.1578579762688188No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGTTGGG15390.15533467102155507No Hit
ACGTAGCAGTGACTGGTATGGGTCACCTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCACCATCCC14980.15119645041604257No Hit
CTGCTACGTCATCCTTCGCACAGTAATAAACGGCCGTGTCCTCGGCTCTC14930.15069178936658983No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14930.15069178936658983No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14720.14857221295888828No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14690.14826941632921664No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14590.14726009423031117No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14480.14614983992151512No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14430.14564517887206235No Hit
CCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAGGTGACCCATAC13590.13716687324125623No Hit
CTCTAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATGGCATAGCTGC13520.1364603477720224No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13500.1362584833522413No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13280.13403797473464923No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13260.1338361103148681No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGGTGCTGCTGTCCCACGCCTGACAGTAATAGT13230.1335333136851965No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12860.12979882191924616No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12850.1296978897093556No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12800.12919322865990285No Hit
CCCCTGTGTTGGTCATCTATCAAGATACTAAGCGGCCCTCAGGGATCCCT12790.1290922964500123No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACCCATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT12790.1290922964500123No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12740.12858763540055956No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12720.12838577098077847No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12630.12747738109176351No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12470.12586246573351473No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12220.123339160486251No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12190.12303636385657937No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACCC12130.12243077059723606No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGGTGCTGCTGTCCCACGCCTGA11890.1200083975598629No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACCCATTATGTCTTCGGAACTG11870.11980653314008179No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGGTGCTGC11760.11869627883128576No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT11610.11718229568292753No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11570.11677856684336532No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC11520.11627390579391259No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11440.11546644811478819No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11380.11486085485544488No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11330.11435619380599214No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11270.11375060054664886No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11250.11354873612686775No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT11130.11233754960818115No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11110.11213568518840006No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGGCCA10960.11062170204004182No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGGTTCCCTGG10750.10850212563234028No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT10620.10719000690376315No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10580.10678627806420095No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG10520.10618068480485766No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG10430.10527229491584272No Hit
GTCTGAGAGCCGAGGACACGGCCGTTTATTACTGTGCGAAGGATGACGTA10400.10496949828617108No Hit
GGCCAGTCCCCTGTGTTGGTCATCTATCAAGATACTAAGCGGCCCTCAGG10340.10436390502682778No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG10300.1039601761872656No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC10250.10345551513781286No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG10240.10335458292792228No Hit
GTGTTGGTCATCTATCAAGATACTAAGCGGCCCTCAGGGATCCCTGAGCG10220.1031527185081412No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA10120.1021433964092357No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10080.1017396675696735No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10010.10103314210043966No Hit
GGGTTCCCTGGCCCCAGAAGTCAAGGTGACCCATACCAGTCACTGCTACGTCATCCTTCGCACAGTAATAAACGG9930.10022568442131527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG150.002446110168.34595
TACGTGT300.003095586864.3997470
TTGCGTA200.006164579754.1194666
TCGTATG652.1100277E-1047.8257440
CTACGCT1450.042.5181728
TCTCGTA901.8189894E-1242.141338
ATCTCGT1000.041.335137
CGTGCGA350.001191926840.42600358
GTGGTAT1700.040.207531
TACGCTG1550.039.79720729
GTAATGC350.001411625439.0587431
CACGAGA1700.038.2087321
CCCACGA1700.038.2010119
CGTATGC1009.094947E-1238.0298241
TCTCCGA1800.037.97186311
GTGTTAG557.887136E-637.2833441
GTATCAA22550.036.5255551
ACGCTGC1700.036.2985530
CGAGACT1800.036.0896623
CGCTGCA1750.035.29361331
Produced by FastQC (version 0.11.5)